方法采用多巴色素法，底物为L-多巴，测定山慈姑及其伪品提取物对酪氨酸酶的激活作用；
MethodsTyrosinase activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reactiom mixture was measured by spectrophotometer.
酪氨酸酶有催化氧化l -酪氨酸及L -多巴生成黑色素的作用。
Tyrosinase performs action for catalytic oxidation of L-tyrosine and L-dopa to form melanin.
多巴氧化酶法在几种方法中是最适合观察黑色素细胞数量和黑色素颗粒的方法。
DopA is the most suitable method to observe the quantity of melanocyte and melanin granules.
多巴染色及电镜观察结果表明转染了反义重组体的B16细胞其黑色素颗粒的含量明显低于对照组。
The results of Dopa staining and electronic microscopy showed that the production of melanin also decreased significantly compared with control B16 cells.
多巴染色及电镜观察结果表明转染了反义重组体的B16细胞其黑色素颗粒的含量明显低于对照组。
The results of Dopa staining and electronic microscopy showed that the production of melanin also decreased significantly compared with control B16 cells.
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