寡核苷酸探针的标记非常重要。
假设你在用32核苷酸长度的寡核苷酸工作。
Suppose you are working with oligonucleotides 32 nucleotides in length.
目的：利用寡核苷酸芯片检测SARS病毒。
Objective: To detect SARS virus by hybridization in oligonucleotide array.
本发明涉及用于调节靶RNA活性的寡核苷酸。
The present invention relates to regulate activity of target RNA oligonucleotides.
目的：建立寡核苷酸膜芯片技术检测SARS病毒。
Objective: To set up an oligonucleotide membrane chip technique for detecting SARS virus.
方法设计一对寡核苷酸引物，其上游引物为突变引物。
Methods A pair of primer was designed and a mismatch nucleotide was introduced in its upstream primer.
结论通过连接酪胺碘标记寡核苷酸法是比较成功的方法。
Conclusions The radioiodination method by conjugating the oligonucleotide with tyramine was very successful.
部分样品的随机测序结果与寡核苷酸阵列杂交结果一致。
Random DNA sequencing result was identical to the results of oligonucleotide array.
目的评价生存素反义寡核苷酸在荷肝癌小鼠中的治疗作用。
Objective To evaluate the therapeutic effect of antisense oligonucleotide targeting Survivin in the treatment of tumor-bearing nude mice.
许多重要的、用天然寡核苷酸无法实现的新应用也应运而生。
Important new applications that could not be performed using natural oligonucleotides have emerged.
目的：筛选出高效特异而无毒的抗hbv反义寡核苷酸片段。
Objective: to screen the high performance, specific and nontoxic anti HBV antisense oligonucleotide fragment.
结果PCNA反义寡核苷酸可明显抑制BT325细胞的生长；
Results PCNA antisense oligodeoxynucleotides could significantly inhibit the growth of BT325 cells line.
目的合成异核苷掺入寡核苷酸并考察它们与互补序列的结合能力。
AimTo synthesize ribonucleoside-incorporated oligonucleotides and investigate their binding abilities with complementary sequences.
方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片。
Methods Oligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed.
MTT法检测加入反义寡核苷酸后对转染的包装细胞生长的影响。
MTT was used to detect the cell growth of the packaging cells.
结论：EAAC 1反义寡核苷酸对急性脑缺血损伤有神经保护作用。
CONCLUSION: EAAC1 antisense oligonucleotide has the neuroprotective effect against acute cerebral ischemia injury.
目的观察vegf反义寡核苷酸对膀胱癌细胞VEGF表达的抑制情况。
Objective To study inhibition of VEGF expression in human bladder carcinoma cells by antisense oligonucleotides.
依其结构，DNA芯片可分为两种形式，DNA阵列和寡核苷酸微芯片。
According to the structures, DNA chip can be divided into two variants, DNA array and oligonucleotide microchip.
结果VEGF反义寡核苷酸对膀胱癌细胞的VEGF表达有明显的抑制作用。
Results VEGF antisense oligonucleotides obviously inhibited VEGF expression in human bladder carcinoma cells and proliferation of endothelial cells.
目的：利用纳米载体增强端粒酶反义寡核苷酸对肝脏肿瘤细胞生长的抑制作用。
Objective: To investigate the growth inhibition of antisense oligonucleotides of telomerase on liver tumor cell enhanced by albumin nanoparticles.
目的探讨超声介导脂质体微气泡转染反义寡核苷酸ha- 2741的有效性。
Objective To explore the efficiency of transfection of antisense oligonucleotides HA-2741 by ultrasound mediated liposome microbubbles.
紫外交联对大片段DNA固定率影响显著，但对氨基修饰的寡核苷酸影响不显著；
Ultraviolet crosslinking greatly affected the attachment rate of a large fragment of DNA, but did not noticeably affect the attachment rate of the amino-modified oligonucleotides.
成功的反义显像要求反义寡核苷酸易被细胞摄取、耐核酸酶、杂交稳定、标记简单。
The requirements for ideal antisense imaging include easy intakeing by cells, convenient labelling, stable hybridization and the tolerance against nuclease.
重要的是要注意，不过，在不同的肌营养不良基因突变可能需要不同的寡核苷酸药物。
It is important to note, however, that different mutations in the dystrophin gene could require different oligonucleotide drugs.
用于诊断流感A病毒的H5和N1基因的存在的寡核苷酸，其用途，检测方法和试剂盒。
Oligonucleotides, use thereof, detecting method and kit for diagnosing the presence of H5 and N1 genes of the influenza a virus.
目的：探讨反义寡核苷酸对胆囊癌细胞端粒酶活性的影响及对胆囊癌细胞生长增殖的作用。
Objective: To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC-SD cell.
利用卟啉的1-羟基苯并三氮唑活性酯可将卟啉基团直接修饰在商品化的寡核苷酸片断上。
The porphyrin moiety was modified on the commercial oligonucleotides through the active 1-hydroxybenzotriazole esters of the porphyrins.
采用DAPI染色技术观察hpk1反义寡核苷酸对OGD诱导的海马神经元凋亡的作用。
DAPI staining was used to investigate the role of HPK1 antisense oligodeoxynucleotides in hippocampus neuron death induced by OGD.
结论：在毛细管dna传感器构建中，溴化乙锭可作为荧光标记物对模式寡核苷酸进行检测。
CONCLUSION: In the construction of capillary DNA biosensors, EB can be taken as the fluorescent marker to detect the target oligonucleotide.
结论：在毛细管dna传感器构建中，溴化乙锭可作为荧光标记物对模式寡核苷酸进行检测。
CONCLUSION: In the construction of capillary DNA biosensors, EB can be taken as the fluorescent marker to detect the target oligonucleotide.
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