MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.
主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。
MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.
主要观察指标：形态观察、增值率测定、碱性磷酸酶染色、细胞基质钙含量测定、茜素红染色显示矿化结节数。
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