The air ambulance service operates helicopters from Marden in Kent and Dunsfold in Surrey.
The Great North Air Ambulance Service later said he had died "despite everyone's best efforts".
Mr Barnes also flew for the Derbyshire, Leicestershire and Rutland Air Ambulance Service.
An air ambulance service is teaming up with a specialist emergency team to treat children around England.
The Children's Air Ambulance Service helicopter, based at Coventry Airport, will initially be used to transport specialist paediatric medical staff.
However opponents, including the Dorset and Somerset Air Ambulance service, say the rebrand is causing confusion among people making donations.
The Coventry-based charity became The Air Ambulance Service (TAAS) after launching a national children's service but is yet to transport any youngsters.
Chief executive of the air ambulance service Adrian Bell said he expected between one and four callouts across the four counties per night.
The air ambulance service covering Kent, Surrey and East and West Sussex has been given the go-ahead to operate 24 hours a day.
The charity works with the Scottish Air Ambulance Service and the new aircraft will be available to respond to routine 999 emergency calls.
The charity has recently rebranded itself as The Air Ambulance Service, and plans to offer a new national service using one helicopter to cover England.
Kevin Hodgson, director of operations at the Great North Air Ambulance Service, worked alongside Mr Barnes for several years, flying on life-saving missions across the North.
It has also taken over the Children's Air Ambulance service.
It launched the national Children's Air Ambulance Service in October.
Orekunrin and her family immediately began looking for an air ambulance service to rapidly transport the girl, a sickle cell anemia sufferer, to a more suitable healthcare facility.
Nick Bourne, the leader of the Welsh Conservatives, said that, despite certain failures in the ambulance service, the assembly government should look to invest in a valued air ambulance service.
Bill Sivewright, chief executive of the Dorset and Somerset Air Ambulance, said the confusion over the Air Ambulance Service lies with whether it provides a national or a more local service.
The Children's Air Ambulance Service is part of the Air Ambulance Service which also looks after Warwickshire and Northamptonshire Air Ambulance at Coventry Airport as well as the Derbyshire, Leicestershire and Rutland Air Ambulance.
Although the new air ambulance will be funded by SCAA, the aircraft will be operated by the Scottish Ambulance Service alongside their own aircraft.
"It is presenting itself as a nationwide service now when in fact only the Children's Air Ambulance part of that organisation actually has a nationwide role, " he said.
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