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Using their least conservative assumptions, the researchers found that less than 2% of the globular clusters should contain intermediate mass black holes today.
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"We were preparing for a long, systematic search of thousands of globular clusters with the hope of finding just one black hole, " said Dr Maccarone.
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Led by George Daley of Children's Hospital in Boston, the researchers began their process by culturing mouse embryonic stem cells to form globular clusters called embryoid bodies.
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Scientists had thought that medium black holes might lie hidden among millions of stars in globular clusters, which sit within galaxies containing hundreds of billions of stars.
MSN: Existence of middleweight black holes nearly ruled out
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Globular clusters are among the oldest structures in the Universe.
BBC: NEWS | Science/Nature | Black hole found in ancient lair
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These stellar-sized black holes are common within globular clusters.
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Such black holes ranging in size from 1, 000 to 10, 000 times the mass of the sun should sit inside globular clusters like scaled-down versions of galactic black holes at least in theory.
MSN: Existence of middleweight black holes nearly ruled out
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"Some theories say that small black holes in globular clusters should sink down to the center and form a medium-sized one, but our discovery suggests this isn't true, " said Daniel Stern, an astrophysicist at NASA's Jet Propulsion Laboratory in Pasadena, Calif.
MSN: Existence of middleweight black holes nearly ruled out
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The globular star clusters that surround the Milky Way also seem to come in two age groups.
ECONOMIST: Stellar origins