For her experiment she studied the inheritance of three traits: a maroon coloured leaf marking, a white V-shaped leaf mark pattern, and the production of hydrogencyanide in damaged leaves, which is thought to be a defence against predators.
Having harvested and germinated seeds from her hand pollinated plants, Minushika scored those seedlings for presence or absence of the red fleck and white V leaf marks and assayed for a specific genetic precursor and enzyme need for the production of hydrogencyanide.