At some point, my girlfriend's mother told me about fugu puffer fish, which is considered a delicacy in Japan how there is a short season for it, and if it isn't prepared exactly right, a bite can kill you.
By contrast, Jean Weissenbach and his colleagues at Genoscope in France made their estimate by comparing the raw human sequence now available (about 60% of the total when their paper was written) with that of a pufferfish.