He was dismissed in the Copa del Rey against Valencia after receiving two yellow cards.
It is Barcelona's second trophy this season after they secured the Copa del Rey in midweek.
Barcelona's Mundo Deportivo newspaper screamed, "No Excuses, " on its front page after the 3-1 humbling against Real in the Copa del Rey.
The home team have not won a trophy in 14 years, but are still competing on two fronts as they face Sevilla in the Copa del Rey final.
In Spain , Real Madrid booked its place in the last four of the Copa del Rey, despite being reduced to nine men during the 1-1 draw at Valencia.
Their loss to Real Madrid in the Copa del Rey might even lead some pundits to put them behind Real Madrid as the second most likely Spanish side to win the tournament.
Those men had been through a grueling season in which their clubs went down to the wire not just in the domestic championship but in the Copa del Rey and Champions League, as well.
Real sits 11 points behind league leader and arch rival Barcelona in the title race, while it faces city neighbor Atletico Madrid in the final of the Copa del Rey, the country's top domestic cup competition.
Trailing Barcelona by 11 points in the Spanish League with five games left, Real's only realistic hope for a title this season is when it hosts Atletico Madrid in the Copa del Rey final on May 17.
Real currently top Spain's La Liga ahead of arch-rivals Barcelona, but their exit from the Champions League mirrors a humiliating defeat to third-flight Alcorcon in the Copa del Rey earlier this season, with Pellegrini again placed in the spotlight.
After a season that failed to yield any silverware -- while arch-rivals Barcelona claimed a remarkable treble of the Copa del Rey, La Liga and Champions League trophies -- the 62-year-old businessman and former politician Perez was a popular choice.
The 22-year-old Messi helped the Catalan giants to the Spanish La Liga and European Champions League titles as well as the Copa Del Rey, while his Argentina side scraped into the World Cup finals after finishing fourth in their South American qualifying campaign.
Garrido has been appointed despite "The Yellow Submarine" being linked with former Getafe coach Michael Laudrup, with the team 10th in La Liga after last year's impressive fifth placing and having been knocked out of the Copa Del Rey by second division Celta Vigo.
He has divided opinion at the Santiago Bernabeu after being unable to follow up last season's title, or deliver Real's long-awaited 10th European title -- and the Copa del Rey final defeat against Atletico Madrid was the last straw as he agreed to leave by mutual consent.
It's also still in the running for another three titles this season: Barcelona has reached the final of the Copa del Rey, the semifinals of the Champions League and is currently in second place in Spain's Liga, though catching Real Madrid would be very difficult.
Spain international Casillas has not played for Real since being injured in January, but in his absence the reigning Spanish champions have been on a fine run, beating Barcelona in the semifinals of the Copa del Rey and in their 'El Clasico league clash Saturday, winning 2-1.
Real were beaten by their arch-rivals Barcelona to both the La Liga title and the domestic cup, the Copa Del Rey, before witnessing an unprecedented treble when the Catalans beat England's Manchester United 2-0 to win the Champions League for the third time and cap a fantastic 2008-09 season.
But Barcelona could still end the season as winners of the Champions League and Copa del Rey.
Toure feels Barcelona are starting to feel the strain of playing fast-paced football with trying to clinch a third European Cup, win the Spanish league and the Spanish Cup (Copa del Rey).
Ibrahimovic was the top scorer in Serie A last season, while Eto'o was equally prolific as Barca clinched an unprecedented treble after landing the La Liga, Copa del Rey and Champions League crowns.
Meanwhile, Barcelona was held to a 2-2 draw in the first leg of its Copa del Rey quarterfinal by 10-man Malaga at Camp Nou.
Ramos, who won the Uefa Cup twice with Sevilla as well as the Uefa Super Cup and Copa del Rey, has turned around Tottenham's form and fortunes since replacing Dutchman Martin Jol.
Barcelona carried off the Spanish title, Champions League and Copa del Rey last season but Ronaldo played down any suggestion that Real could immediately replicate their bitter rivals' achievement.
Atletico stayed third after the defeat, but can still win a trophy this season by beating city rivals Real in next Friday's Copa del Rey final.
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