并应用HE染色和免疫荧光技术分析正常和HD小鼠胰岛形态学差异。
Further, he staining and immunofluorescence technique were used for morphometric analysis of islets in normal and HD mice.
方法：采用受体放射配基结合分析、受体-DNA结合分析及免疫荧光技术。
Methods: receptor radioligand binding assay, receptor DNA binding assay and immunofluorescence methods were used.
目的：观察超声成像及间接免疫荧光技术在骨髓基质细胞移植治疗缺血心肌病方面的评估价值。
AIM To observe the evaluation value of ultrasonic imaging and indirect immunofluorescence technique in the treatment of ischemic cardiomyopathy with marrow stroma cells MSCs transplantation.
介绍了应用激光扫描共聚焦显微镜，结合免疫荧光技术研究卵母细胞和早期胚胎细胞骨架的方法。
This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique.
方法采用细胞免疫荧光技术通过免疫荧光显微镜和激光共聚焦显微镜观察HPK1是否在大鼠脑细胞中存在；
Methods Immunofluorescence technique was used to investigate whether there was HPK1 in hippocampus neuron under the fluorescence microscope and the confocal microscopy.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
目的：利用抗脱氧雪腐镰刀烯醇多克隆抗体及其人工抗原，采用时间分辨免疫荧光技术建立间接竞争脱氧雪腐镰刀烯醇时间分辨免疫检测方法（DON-TRFIA）。
Objective:An indirect competitive time-resolved fluoroimmunoassay(TRFIA) was established using anti-DON polyclonal antibody and coating antigen DON-BSA to detect deoxynivalenol in cereal.
这就经常意味着增菌培养试验需要优先于免疫荧光显微技术。
This usually meant that enrichment culture techniques were required prior to immunofluorescence microscopy.
并采用免疫荧光抗体染色技术对5例dmd,2例BMD肌细胞膜上抗肌营养不良蛋白的表达观察分析，以2例正常人的肌组织作为对照。
The patients from 5 DMD and 2 BMD were detected by immunofluorescence technique for analyzing dystrophin located in muscle cell membrane, compared with 2 normal males.
本文目的是探讨耳蜗组织免疫荧光染色及激光共聚焦显微镜技术的应用。
The purpose of this article is to explore The Organization of cochlear immunofluorescent staining and laser scanning confocal microscope technology applications.
方法：采用细胞免疫荧光染色技术和流式细胞术。
Methods Flow cytometry and immunofluorescence staining technique were used.
探讨以计算机图像处理技术将普通荧光显微镜采集的同视野单标记免疫荧光图像合成为双标记图像的方法。
The present study investigated the methodology of synthesizing two single-staining immunofluorescence images into one overlapped double-stained image by using computerized image-processing techniques.
方法免疫组织化学和免疫荧光双标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
结论视网膜血管消化铺片联合免疫荧光组织化学技术是可行的，为视网膜血管性病变的研究提供了重要的途径。
Conclusion retinal digest preparations combined with immunofluorescence. was feasible and provided an important method for the studies of retinal vascular diseases.
方法：免疫荧光组织化学双重标记技术。
METHODS: Immunofluorescence histochemical double-staining method was used.
运用免疫荧光和蛋白印迹技术检测了5种食管癌细胞系中NGAL的表达。
The expression level of NGAL in 5 esophageal carcinoma cell lines was also examined by immunofluorescence and western blot.
用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；
The expression of cell surface-bound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis.
方法：荧光金(FG)逆行追踪结合5 HT免疫荧光组织化学染色的双标技术。
METHODS: Fluoro Gold (FG) retrograde tracing combined with serotonin (5 HT) immunofluorescence histochemical staining method was used.
方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。
Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
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