GJB2基因敲除小鼠在胚胎期致死。
GJB2 gene knockout mice led to death during embryonic period.
其结果是，基因敲除基因的老鼠吃比正常老鼠更多。
The upshot was that the knockout mice ate considerably more than the normal animals.
另外，该基因敲除小鼠可以作为研究躁狂症的模型。
In addition, the knockout mice might be useful as models to study mania.
结论套环能加速载脂蛋白e基因敲除小鼠颈总动脉斑块形成。
Conclusions Perivascular common carotid collar placement can accelerate atherosclerotic plaque formation in apolipoprotein E-knock out mice.
为了更具体的验证这个途径，研究者把小鼠的DDAH基因敲除。
To examine this pathway in more detail, the researchers deleted the DDAH gene in mice.
目的研究蚤休水提液对载脂蛋白E基因敲除小鼠晚期斑块的影响。
Objective: To study the effects of water extract from Paris Polyphylla Smith on advanced plaques in ApoE gene knockout mice.
PTEN基因敲除的小鼠是一种慢性肝损伤模型，最终导致肝癌。
The PTEN knock-out mouse is one model of chronic liver injury that ultimately leads to liver cancer.
雌性野生种小鼠的丙氨酸转氨酶是基因敲除鼠的4倍，这提示肝损伤的存在；
Female wild-type mice on an ethanol-rich diet had 4 times as much alanine aminotransferase (ALT), indicating liver injury, than mice lacking the osteopontin gene.
Oliver现在研究PIDD基因敲除的肿瘤细胞，看看是否它的缺失阻碍药物耐药性。
Oliver is now studying tumors in which the PIDD gene has been knocked out, to see if its absence hinders drug resistance.
其次，D3R基因敲除小鼠研究提示，正常生理状态下D3R仅表现辅助功能；
The D3R knock-out mice have also been generated. The results from these mice show D3R plays a secondary role in physiological condition.
论述了基因敲除的基本原理、几种常用技术及该项技术在微生物育种方面的应用。
In this paper, the basic principles of gene knockout, several common techniques and their application in microbial breeding were introduced.
基因敲除技术的广泛应用，极大地推动了后基因组时代对于基因功能研究的进程。
The widespread use of gene knockout technology promotes the course of the research of gene function in post genomic era greatly.
并且对基因敲除进行时空的调控，并成为脊椎动物成体中研究基因功能的重要手段。
Conditional gene knock-out has become an important means to research gene function in vertebrates.
并且对基因敲除进行时空的调控，并成为脊椎动物成体中研究基因功能的重要手段。
Moreover, it regulates gene knock-out spatially and temporally. Conditional gene knock-out has become an important means to research gene function in vertebrates.
同时，课程也将对RNA干扰和基因敲除等重要的实验技术的原理及应用作专题介绍。
In addition, several useful laboratory techniques, e. g. small RNA interference and gene knockout are comprehensively introduced during the lecture.
研究人员使用经过基因敲除的小鼠来做实验，这些小鼠的舌头中缺乏特定的味觉受体。
The researchers also conducted experiments using knockout mice lacking specific taste receptors in their tongues.
另外，该基因敲除小鼠可以作为研究躁狂症的模型。现在还没有可用于躁狂症的动物模型。
In addition, the knockout mice might be useful as a model to study mania, as there is no other animal model available yet.
Saltiel说：“在这项研究中，基因敲除的小鼠并没有比对照组小鼠进行更多的运动。”
"The knockout mice are not exercising any more than the control mice used in the study. They're just burning more energy," Saltiel said.
尽管整体心室壁厚度下降，但是特定区域的形态学改变和基因敲除小鼠的心室壁粘液瘤的形成一致。
Although heart wall thickness was reduced overall, specific areas exhibited morphological changes consistent with myxomatous degeneration in the walls of knockout hearts.
最近的研究表明，COX-2基因敲除小鼠的生殖缺陷主要依赖小鼠的遗传背景，与小鼠的品系相关。
However, it was recently shown that the reproductive defects of COX-2-deficient mice mainly depend upon genetic background of the mouse strains.
结果肾缺血-再灌注导致BUN及血清肌酐浓度明显升高，HSF1基因敲除导致二者上升更加明显。
ResultsIschemia and reperfusion caused renal injury as indicated by the increase of BUN and serum creatinine levels, which was exacerbated by HSF1 knock out.
我们的研究团队在2004年对基因敲除小鼠进行的研究发现，这类小鼠的神经功能丧失部分地减少了。
Our group has shown in 2004 that in knockout mice that are partially protected from a loss in nerve function.
以前的研究表明，COX - 2基因敲除的小鼠是不孕的，并且排卵、受精、着床以及蜕膜化均出现异常。
A previous study showed that COX-2-deficient females are infertile with abnormalities in ovulation, fertilization, implantation and decidualization.
运用敲除、条件基因敲除或者过度表达的方法来改变发育过程中的基因表达，探讨在系统中出现的这些变化。
By changing gene expression patterns during development through knockouts, conditional knockouts, or overexpression, the changes in the system can be explored.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
小鼠ptp - 1b基因敲除及反义核苷酸治疗实验表明PT P - 1b是治疗糖尿病和肥胖症的潜在靶标。
Recent PTP-1B gene knock-out studies and anti-sense oligonucleotide (ASO) treatment in mice have identified PTP-1B as a potential target for chemotherapy of diabetes and obesity.
在AEP基因敲除的小鼠实验中，该药物及人为中风所导致的DNA损伤与脑细胞死亡均比非基因敲除小鼠程度减轻。
In mice genetically engineered to lack AEP, both the drug and an artificial stroke resulted in reduced DNA damage and less brain cell death than in regular mice.
结论DPR基因敲除型小鼠脑中组胺能神经活性显著降低，这一变化可能是该种小鼠尚能维持正常睡眠的代偿机制之一。
Conclusion The activity of the histaminergic system decreased and the decrease might be one of the compensatory mechanisms for maintenance of the normal sleep in the null mice lacking DPR.
本文简述了基因敲除技术的基本原理、打靶策略、筛选机制，在动植物和微生物中常用的基因敲除方法以及基因敲除的应用。
This paper introduced the principle, the targeting strategies, the screening mechanism and the commonly method in animals, plants and micro-organisms of gene knockout.
本文简述了基因敲除技术的基本原理、打靶策略、筛选机制，在动植物和微生物中常用的基因敲除方法以及基因敲除的应用。
This paper introduced the principle, the targeting strategies, the screening mechanism and the commonly method in animals, plants and micro-organisms of gene knockout.
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