和传统的凝胶技术相比,毛细管等电聚焦(CIEF)是一种蛋白质的快速分离技术。
Capillary isoelectric focusing (CIEF) is a more rapid technique for separating proteins that the traditional gel-based technique.
讨论了毛细管等电聚焦中所涉及的问题,如分离机理、电渗、迁移方法、检测器及其应用。
Separation mechanism, manipulation of electroosmosis, mobilization methods, detectors and some problems associated with the determination of samples are discussed.
蛋白质首先在毛细管等电聚焦过程中根据它们等电点的差异得到分离,然后被电喷雾质谱鉴定。
Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.
蛋白质首先在毛细管等电聚焦过程中根据它们等电点的差异得到分离,然后被电喷雾质谱鉴定。
Proteins were separated and resolved in CIEF according to their differences in isoelectric point (pI), and then characterized by ESI MS.
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