并用红外光谱、水合茚三酮反应、含氮量分析等方法验证接枝反应的进行。
The proof of grafting was established by IR spectra, ninhydrin test, nitrogen estimation and other analytical methods.
方法：采用氨基酸的游离氨基与水合茚三酮产生显色反应，在一定范围内，其颜色的深浅与氨基酸的含量成正比。
Methods: color reaction of free amino acids with ninhydrin was adopted, since, in certain extent, the color depth is correlated with the amino acid content.
方法在碱性条件下，以（水合）茚三酮乙醇溶液为显色剂，采用比色法测定，检测波长为568。
Methods Alendronate sodium was determinated by colorimetry and ninhydrin ethanolic solution was used as chromogenic agent in alakaline condition and detection wavelength was at 568.
方法在碱性条件下，以（水合）茚三酮乙醇溶液为显色剂，采用比色法测定，检测波长为568。
Methods Alendronate sodium was determinated by colorimetry and ninhydrin ethanolic solution was used as chromogenic agent in alakaline condition and detection wavelength was at 568.
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