最终,在机器后面的基本概念是相对简单:使用五个交替在硼硅玻璃培养皿用PDMS层,一个活动大桶载体上。
Ultimately, the basic concept behind the machine is relatively simple: using five alternating petri dishes in borosilicate glass with a PDMS layer, on a moving VAT carrier.
生物医学研究者们越来越认识到在二维培养皿和玻璃载玻片上培养细胞的局限性。
Biomedical researchers have become increasingly aware of the limitations of growing living cells in coated, two-dimensional Petri dishes and glass slides.
玻璃。装细菌用玻璃器皿。“陪氏”培养皿。
脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里,剪成两瓣,用无菌玻璃的边缘挤压,获得单细胞悬液。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里,剪成两瓣,用无菌玻璃的边缘挤压,获得单细胞悬液。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
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