此起事件为居民家中聚餐引起的副溶血性弧菌食物中毒。
This event of vibrio parahaemolyticus food poisoning was due to the food pollution in residents' home dinner.
目的运用脉冲场凝胶电泳技术和荧光PCR技术对成都市2008-2009年分离的副溶血性弧菌进行分子分型和毒素基因检测,分析感染来源。
The purpose of this study is using pulsed field gel electrophoresis(PFGE) and PCR typing to analyze Vibrio parahemolyticus isolated from outbreaks during 2008-2009 and to trace the infection source.
前言:目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。
Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus, develope a pandemic group-specific multiplex PCR assay for base unit.
结果运用群特异多重PCR法可特异性的扩增出当前流行群副溶血性弧菌的目的基因片段,可大大缩短鉴定时间。
Results The aim gene fragment can be amplified by a multiplex PCR assay, it largely reduce the time of identification.
具体结论如下:研究了超高压处理对副溶血性弧菌致死率、细胞超微结构、细胞膜通透性、细胞ATP酶活性的影响,为探讨微生物的高压致死机理提供依据。
The results were as followed. The effect of ultra high pressure treatment on the lethal rate, the ultra-structure, membrane permeability, ATPase activity of Vibio Parahaemolyticus were studied.
结论:食品中的大流行株及致病株的出现是副溶血性弧菌食物中毒事件发生的主要原因。
Conclusion: the pandemic strains and pathogenic strains in food chains were the main reason that caused foodborne breaks in China.
结果:所检测的小龙虾和呕吐物中副溶血性弧菌均为不致病性副溶血性弧菌。 小龙虾分离菌株对替卡西林和头孢噻吩耐药。
Results:The two strains of Vibrio parahaemolyticus isolates were non-pathogenic, and the strain isolated from small lobster was tolerant to ticarcillin and cephalothin.
结果:所检测的小龙虾和呕吐物中副溶血性弧菌均为不致病性副溶血性弧菌。 小龙虾分离菌株对替卡西林和头孢噻吩耐药。
Results:The two strains of Vibrio parahaemolyticus isolates were non-pathogenic, and the strain isolated from small lobster was tolerant to ticarcillin and cephalothin.
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