自1983年我国学者创立花粉管通道法以来，在多种农作物中得到应用。
The method of pollen tube pathway was formally established in 1983. Since then, it has been widely used in crop genetic improvement.
实际转化操作表明，子房滴注转化法的PCR阳性率（4.5%）要高于花粉管通道法（2.0%）。
More PCR positive plants could obtain by ovary-drip (4.5%) than that by pollen tube pathway (2.0%), when transformation was conducted.
采用花粉管通道法遗传转化技术，将慈姑蛋白酶抑制剂基因API导入水稻品系“9311”，获得了转基因植株。
Transgenic rice plant was obtained by transferring the Arrowhead Proteinase Inhibitor (API) gene into rice line "9311" by pollen-tube pathway method.
结果表明 ：几丁质酶对镰刀菌引起的西瓜枯萎病有一定的抑制作用 ，利用花粉管通道法直接导入西瓜活体植株的技术是可行的。
The results indicated that the chitinase had certain inhibition effect on Fusarium oxysporum, and the method of pollen tube pathway could be successfuly used in watermelon transformation.
结果表明 ：几丁质酶对镰刀菌引起的西瓜枯萎病有一定的抑制作用 ，利用花粉管通道法直接导入西瓜活体植株的技术是可行的。
The results indicated that the chitinase had certain inhibition effect on Fusarium oxysporum, and the method of pollen tube pathway could be successfuly used in watermelon transformation.
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