通过双荧光素酶报告基因测定法测定了IGFBP-3对由三碘甲状腺素刺激的生长激素启动子活性的影响。
The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.
方法采用荧光素酶报告基因方法。
相对荧光素酶法检测转录激活活性。
Transcriptional activation activity of variant was evaluated through methods of relative activity of luciferase.
结果获得稳定、高活性的荧光素酶。
同年，克隆荧火虫的荧光素酶也取得成功，这个非常有用。
The cloning of luciferase from fireflies, which also took place in 1985, proved just as useful.
文章就荧光素酶的种类、结构及应用进行了研究。
The species, structure and applications of luciferase were researched.
我们用荧光素酶报告基因的方法，探讨了这个问题。
We addressed this question with the method of luciferase reporter gene.
相对荧光素酶活性法检测GR转录激活功能的变化。
The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.
小鼠还定期注射荧光素，以便荧光素酶能够持续发光。
The mice were also regularly injected with luciferin, so that the luciferase would have the fuel it needed to glow.
对发现的突变，进行荧光素酶报告基因瞬时表达研究。
The reporter gene was used to study the mutation influencing the activity of promoter.
虫荧光素酶稳定性加强，保存时间长，使用更为方便快捷。
Therefore, the luciferase has enhanced stability and long storage time, and can be used more conveniently and rapidly.
相对荧光素酶活性法检测地塞米松作用后GR转录激活功能的变化。
The transcriptional activation function of GR was evaluated through the detection of relative luciferase activity after dexamethasone treatment.
荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。
Luciferase reporter gene technique plays an important role in high throughput screening.
研究者用基因工程的大肠埃希菌细胞生产荧光素酶作为AHL浓度函数。
The investigators used genetically engineered E. coli cells to produce luciferase as a function of AHL concentration.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。
The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.
双荧光素酶报 告载体和突变双荧光素酶报告载体分析证实了它们能够相互作用。
Dual-luciferase reporter vector and mutated dual-luciferase reporter vector analysis validated that they are interacted.
荧光素—荧光素酶反应体系能发出荧光，毒性物质的存在则会抑制荧光的发光强度。
The luciferin-luciferase's reaction system may emit fluorescence, while the toxic substances will suppress the luminous intensity.
核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。
In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.
瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。
The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.
三个突变异种均显示比野生型具有更高的荧光素酶活性，并且V85I突变异种活性最强。
All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity.
最简单的发光反应需要一种叫做荧光素有机分子，在荧光素酶的帮助下，进行被氧气氧化的氧化反应。
The simplest light-producing reactions require an organic molecule, known as a luciferin, to be oxidised by oxygen, a process that is helped along by an enzyme known as luciferase.
创伤后并发器官损害病人的荧光素酶报告基因活性显著高于无器官损害者，其死亡率也较高。
On the other hand, the activity of LUC was higher in the organ damagd patients than that in the non-organ damagd patients, with a high mortality rate.
去年，丘奇的研究团队通过自组装获得了合成核糖体，并产生了荧光素酶，这种蛋白质可以使萤火虫发光。
Though they haven’t published their results yet, last year his team got a synthetic ribosome to self-assemble and produce luciferase, the protein that makes fireflies glow.
结论：成功构建了缺氧应答启动子的荧光素酶报告载体，为进一步研究其缺氧调控作用提供了条件。
CONCLUSION: Luciferase reporter vectors regulated by HRE/CMV promoters were successfully constructed, which makes it possible to further investigate their potency of hypoxia regulation.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。
ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
研究者透露说，在水或食物样品中加入荧光素酶和荧光素可以被用来检测atp，这样就可以确定样品中是否存有细菌或者其他病原体。
Researchers realised that adding luciferase and luciferin to a sample of water or food could be used to detect ATP, and thus determine whether the sample contained bacteria or other pathogens.
在荧光素酶中加入正确的荧光素底物就可以放出荧光，而发出的光子可以被光敏感元件，如荧光探测器或改进后的光学显微镜探测到。
Light is emitted when luciferase is exposed to the appropriate luciferin substrate. Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.
在荧光素酶中加入正确的荧光素底物就可以放出荧光，而发出的光子可以被光敏感元件，如荧光探测器或改进后的光学显微镜探测到。
Light is emitted when luciferase is exposed to the appropriate luciferin substrate. Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.
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