Objective To obtain human rhinovirus 3C protease as a novel tool enzyme for gene engineering.
目的获得重组3C蛋白酶，开发一种新型的基因工程工具酶。
Objective Application of human rhinovirus 14 3c protease as a tool-enzyme for more stringent sequence specificity and efficient cleavage at low temperature.
目的人鼻病毒3c蛋白酶具有酶切物异性强且在低温下仍保持较强酶活性的特点而被用作工具酶。
Conclusion The recombinant GST-3C protease can be not only applied to the cleavage of fusion proteins, but also be used as a tool-enzyme in TAP system containing 3C recognition sequence.
结论GST-3C蛋白酶不仅可以应用于融合蛋白的酶切，而且为引入3C蛋白酶识别位点的串联亲和纯化（TAP）系统提供又一种酶切工具。
Conclusion The recombinant GST-3C protease can be not only applied to the cleavage of fusion proteins, but also be used as a tool-enzyme in TAP system containing 3C recognition sequence.
结论GST-3C蛋白酶不仅可以应用于融合蛋白的酶切，而且为引入3C蛋白酶识别位点的串联亲和纯化（TAP）系统提供又一种酶切工具。
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