Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST -MBD4融合蛋白。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST -MBD4融合蛋白。
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