To improve the method of myoblast cell culture in vitro.
改进体外成肌细胞培养方法。
Purpose: For preparing the new biosyntbetic vascular graft, endothelial cell culture in vitro was studied.
目的：为制备新型复合血管，对内皮细胞的体外培养进行研究。
OBJECTIVE: Animal cell culture medium is an important factor of cell culture in vitro, which can influence the cell growth.
目的：动物细胞培养基是体外细胞培养的重要因素，能够影响细胞生长。
AIM: to investigate differentiation of CD34 + cells in human umbilical blood into eosinophils under the condition of cell culture in vitro.
目的：探讨人脐血cd 34 +细胞在体外培养条件下向嗜酸粒细胞分化的规律。
Methods: We built the pancreatic cancer cell group HS 766t and human endothelium cell in the umbilical vein by the way of cell culture in vitro.
方法：采用细胞培养的方法建立稳定传代的胰腺癌hs 766t细胞系，体外培养人脐带静脉内皮细胞。
The effects of sodium arsenite on certain Biochemical Function in heart of mice were explored by myocardial cell culture in vitro and whole animal experiment.
通过体外心肌细胞培养试验和动物实验，探讨了砷对心脏某些生化功能的影响。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
STUDY SELECTION: Totally 45 literatures were selected about anticarcinogenic effect of CLA using animal experiments, cell culture in vitro, mechanisms and synthetical methods of CLA.
资料选择：选择共轭亚油酸抗癌的动物实验及体外细胞培养研究，作用机制研究以及人工合成方法等方面的相关文献45篇。
PART I The Improvement of Dendritic Cell Culture Measures in vitroObjective: Improve the measures of dendritic cell culture in vitro, to increase the rate of mature and function of DC.
目的：探讨改善外周血来源的树突状细胞（DC）体外培养的方法，以促进DC的成熟并提高其递呈抗原和激发特异性免疫反应的能力。
We have to do in-vitro cell culture experiments first, prove it in an animal model, and then develop a molecule or protein which will affect the mutated gene product.
我们首先得做一个体外细胞培养实验，在动物模型中证明它的存在，然后开发出一种能影响突变基因产物的分子或蛋白质。
To improve the cell culture method of BTM cells in vitro.
探索并改进体外牛眼小梁细胞的培养方法。
Methods: In vitro culture cell number count assay was adopted.
方法采用体外细胞培养计数法。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.
目的探讨人角膜缘干细胞体外培养后细胞形态学、抗原性和增殖能力的变化。
PURPOSE: 1 To build high pressure cell culture model in vitro of Retinal Ganglion Cells(RGCs) and observe the RGCs effected by pressure .
目的：1建立高压体外培养视网膜神经节细胞模型并观察压力对视网膜神经节细胞的影响；
The present experiment studied the culture methods of swine vascular endothelial cell (SVEC) in vitro.
实验研究了猪血管内皮细胞体外培养方法并对培养的细胞进行了鉴定。
OBJECTIVE: to analyze the qualifications for in vitro culture of embryonic germ cell for the purpose to set the foundation of establishing embryonic germ cell system in vitro.
目的：分析胚胎生殖细胞体外原代培养条件，为胚胎生殖细胞建系奠定基础。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Differentiation of muscle satellite cell under different culture conditions in vitro was also studied.
同时观察不同培养条件下骨骼肌卫星细胞的成肌分化特性。
This article focuses mainly on optimization of human EG cell isolation and in vitro culture system.
本文主要对人类EG细胞分离及体外培养的体系进行优化。
METHODS in vitro methylcellulose semisolid culture was used for the evaluation of clonal formation of K562 cell line. Flow cytometer was used for detecting apoptosis.
方法体外甲基纤维素半固体培养观察各处理组k 562细胞的克隆形成，流式细胞仪评价细胞的凋亡状况。
Objective: To study the localization and in vitro culture of human embryonic primordial germ cell(PGC) during different stages of embryogenesis.
目的：对不同发育阶段的人胚胎原始生殖细胞（PGC）进行定位并比较其体外培养的差异。
The experiment was blastocyst and hatch blastocyst of ICR mouse culture in vitro, and the effect of two methods to the cell counting of mouse embryo was compared.
实验以ICR小鼠体外培养的囊胚及孵化胚胎为材料，比较两种方法对小鼠胚胎细胞计数的效果。
Objective To investigate the feasibility of amplifying big animal chondrocytes in vitro by using rotary cell culture system (RCCS).
目的探讨用旋转细胞培养系统(RCCS)进行大动物软骨细胞扩增的可行性。
Methods in vitro culture cell number count was adopted. Results AGP significantly inhibited the gastric cancer cell.
方法采用体外细胞培养计数法，结果表明，该多糖对胃癌有显著性抑制作用。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
Methods Using dissociated cell culture of rat embryo in vitro.
方法采用大鼠胚胎中脑神经元细胞进行体外培养。
In laboratory research, we observed effect of Huo Shui TAB on the bone marrow micro-environment of immune-mediated aplastic anemia on mice by in vitro culture of bone marrow stromal cell.
实验部分通过兔疫介导的再障小鼠骨髓基质细胞体外培养探索活髓片在骨髓造血微环境方面的作用机理。
In laboratory research, we observed effect of Huo Shui TAB on the bone marrow micro-environment of immune-mediated aplastic anemia on mice by in vitro culture of bone marrow stromal cell.
实验部分通过兔疫介导的再障小鼠骨髓基质细胞体外培养探索活髓片在骨髓造血微环境方面的作用机理。
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