Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500.
方法： 行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；
Cell viability was determined by MTT assay, cell cycle and apoptosis rate were analyzed by flow cytometer.
利用MTT法测定细胞成活率，流式细胞仪测试样品的细胞周期与凋亡率。
Cell viability was examined by methyl thiazolyl tetrazolium (MTT) assay and 50% inhibitive concentration (IC50) of cisplatin was examined also.
四甲基偶氮唑蓝比色法检测细胞存活率并计算顺铂的50%抑制浓度(IC50)。
The cell viability was determined by try pan blue exclusion assay and cell cycle analyzed by flow cytometry, with the ce ll apoptosis assayed by TUNEL method.
应用锥虫蓝染色测定细胞活力、流式细胞计数分析细胞周期、TUNEL分析细胞凋亡。
Cell viability, the change of membrane fluidity, LDH activity in bath and lipid peroxidation injury were investigated by MTT assay, fluorometric technique and spectrophotometric method, respectively.
通过MTT法测定细胞存活率、分光光度法测定细胞LDH漏出率及细胞脂质过氧化水平，采用荧光标记技术研究细胞膜流动性变化。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
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