Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
SDS-PAGE was used to detect the purity of target protein. A higher bioactivity of purified products was shown by the determination of dot-blotting assay and fibrinogen-clotting analysis.
通过SDS - PAGE检测融合蛋白的纯度，采用点杂交和纤维蛋白凝结活性检测分析，显示纯化产物具有较高生物活性。
SDS-PAGE was used to detect the purity of target protein. A higher bioactivity of purified products was shown by the determination of dot-blotting assay and fibrinogen-clotting analysis.
通过SDS - PAGE检测融合蛋白的纯度，采用点杂交和纤维蛋白凝结活性检测分析，显示纯化产物具有较高生物活性。
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