Methods Immunohistochemistry and electrophoretic mobility shift assay (EMSA) were used.
方法采用免疫组化法及凝胶电泳迁移率实验。
First, we carried out Electrophoretic Mobility Shift Assay (EMSA) to investigate the binding difference of SNPs and transcription factors.
首先使用凝胶迁移检测和疾病关联的SNP是否会影响某些转录因子的结合。
Method AP-1 activity of macrophages was detected by using electrophoretic mobility shift assay, after WPG stimulated peritoneal macrophages of SD mice.
方法以WPG刺激SD大鼠腹腔巨噬细胞，采用凝胶电泳迁移分析技术检测巨噬细胞AP - 1的活性。
Moreover, the electrophoretic mobility shift assay analysis demonstrated that the HuH-7 nuclear proteins form a specific protein-DNA complex composed of LS2 motif.
进一步以胶体电泳位移分析法证实，在LS2 区域会形成HuH-7 核蛋白及DNA的复合物。
The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).
用双染免疫组化法检查脾细胞中STAT 5磷酸化与STAT 5抗原的定位。用电泳迁移率变动分析(EMSA)测定STAT 5与DNA探针的结合力。
The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).
用双染免疫组化法检查脾细胞中STAT 5磷酸化与STAT 5抗原的定位。用电泳迁移率变动分析(EMSA)测定STAT 5与DNA探针的结合力。
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