During digestion in the enzyme solution some spontaneous fusion of protoplasts could be observed and the fused protoplasts were generally large, cytoplasmic dense and varied in shape.
在酶的解液中进行消化期间，可以观察到一些原生质体自然融合，融合的原生质体体积大、胞质浓、形状各异。
Fine Gansu enzyme, eats food to shake a kind of side afterward to be able to slow down the digestion process, plays falls the special role.
一精甘酶，随后进食的撼类方能减缓消化过程，起到降特的作用。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
以18天鸡胚睾丸为实验材料，经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Meathod: The experiment adopted density gradient centrifuging to separate PBMC of 44 patients with CHF and 20 normal person and enzyme digestion to separate neonatal rat cardiac myocyte.
方法：密度梯度离心法分离44例CHF患者和20例正常人PBMC，酶消化和差速贴壁法分离乳鼠心肌细胞。
Enzyme digestion was used to detect the viability of fresh tendon, which was 89.26%, and that of vitrification-preserved tendons was 78.49%.
应用酶消法测得新鲜肌腱活性为89.26%，玻璃化法优化组合所保存肌腱的活性为78.49%。
The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
组织块培养法对于原代细胞的培养要优于酶消化法。
Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively.
方法采用酶消化法及酶辅助机械分离法分别分离IPE和RPE细胞。
Red yeast contains many biodegradable starch, protein, pectin, beta-galactosidase enzyme that helps digestion.
红有多种可分解淀粉，蛋白质，果胶，半乳糖的水解酵素，可帮助消化。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
MethodsModified excimer laser surgery, regular excimer laser surgery, and enzyme gradation digestion were used to isolate retinal photoreceptors.
方法应用改良的准分子激光切削法和酶分层消化法来分离纯化视网膜感光细胞层。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
The elementary procedure of this technology contains sample disposal, protein enzyme digestion, in-situ PCR and the detection of sequence copied.
原位PCR技术的基本步骤包括标本处理、蛋白酶消化、原位PCR扩增和扩增后产物的检测。
In addition , the action results of their enzyme preparation were assessed with the digestion test in vitro.
另外，通过体外消化试验评价了三个菌株所产酶制剂的作用效果。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Papaya, rich in papaya enzyme digestion, conduce to the meat protein with absorption.
木瓜中丰富的木瓜酵素，有助于肉类蛋白质的消化与吸收。
The present work revealed that the active oxygen radicals were generated from alfalfa callus cells during cell wall digestion in enzyme solution.
研究发现，分离原生质体的酶解脱壁处理可以诱导苜蓿细胞产生活性氧。
With Berry digest, a special enzyme blend that helps support digestion.
浆果消化，一种特殊的酶，有助于促进消化。
To evaluate its heredity stability, the recombinant virus genome was extracted and digested by Hindlll every 5 generations, and 40 generations later, its enzyme digestion sites almost keep stable.
同时在连续传代40代后每隔5代提取重组病毒全基因组进行酶切鉴定，发现其酶切位点保持不变。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
结论：由于PCR -双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.
而消化的人胚肺组织中细胞形态不一，经继续培养4～5代后，培养物完全由成纤维细胞构成。
Results: LIF gene sequence and vector were verified correctly by enzyme digestion, sequence analysis.
结果经酶切和DNA测序证明LIF基因序列正确，载体构建成功。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
Animal Parade Tummy zyme further supports healthy digestion with an enzyme-rich whole food blend and live probiotics.
同时每片消化酶还富含周全的食物同化物和活性益生菌加健旺康的消化。
The length and the results of restriction enzyme digestion indicate that the amplified products are respected.
各扩增产物长度和限制性酶酶切结果表明均为预期产物。
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