Objective:In order to seek a solution to the extensive burn and other traumatic skin defect and lesion, we have had several studies on the human epidermal cell culture and homoplastic transplantation.
目的：为解决大面积烧伤及其它创伤性皮肤缺损创面覆盖问题，我们对人表皮细胞培养法及同种移植进行了研究。
Method a: Cultured epidermal cell sheets were fixed on the silicone oil gauze. Method b: Direct cryopreservation of the culture chamber containing epidermal cell sheets.
冷冻保存方法是：将表皮细胞培养膜片固定在含硅油纱布上进行冷冻保存(方法a)和将在培养瓶中的表皮细胞培养膜片直接冷冻保存(方法b)。
Methods Epidermal basal cells were cultured with common cell culture technique.
方法以基底细胞作常规细胞培养；
The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
Methods The mixed epidermal cell and lymphocyte culture (MELC) was established, in which auto-keratinocytes were added to mimic the effect exerted by the auto-islets in vivo.
结果自体角质形成细胞可抑制淋巴细胞对同种异体抗原的增殖。
Methods The mixed epidermal cell and lymphocyte culture (MELC) was established, in which auto-keratinocytes were added to mimic the effect exerted by the auto-islets in vivo.
结果自体角质形成细胞可抑制淋巴细胞对同种异体抗原的增殖。
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