This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
MCHR2; eukaryotic expression vector; transfection; gene expression.
MCHR2；真核表达载体；转染；基因表达。
To construct the eukaryotic expression vector of GGT1 and detect its localization in COS7 cells.
构建GGT1重组真核表达载体，观察GGT1在COS7细胞中的定位。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
Objective to construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.
目的建立丙型肝炎病毒(HCV)复合多表位基因的真核表达载体，并在COS7细胞中瞬时表达。
Results rat IL-10 eukaryotic expression vector had been constructed successfully, and had been transfected into rat chondrocyte.
目的构建大鼠il - 10真核表达载体并在大鼠软骨细胞中进行表达。
The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.
实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。
Objective To construct the human G250 eukaryotic expression vector and establish the stable B16 cell line expressing human G250 in mice.
目的构建人G250真核表达载体，建立稳定表达人G250的小鼠黑色素瘤细胞系。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Objective: to establish NK cell receptor NKG2D eukaryotic expression vector and investigate on the cytotoxicity of NK cell line-YT transfected with NKG2D.
目的建立NK细胞受体nkg2d真核表达载体，通过转染NK细胞系yt，初步探讨NKG2D分子对YT细胞系杀伤功能的增强作用。
Objective to construct eukaryotic expression vector of human pancreatic duodenal homeobox 1 (PDX-1) gene, and to detect its expression in NIH3T3 cell lines.
目的构建人胰十二指肠同源盒(PD X - 1)基因的真核表达载体，观察其在NIH3T3细胞中的表达。
AIM: to construct eukaryotic expression vector carrying human antisense VEGF gene and to study its effect on VEGF expression and growth of renal cell carcinoma.
目的：构建反义vegf基因真核表达载体，研究其对肾癌细胞VEGF表达的影响。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
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