Objective To explore the conditions under which fluorescence intensity is determined in confocal laser scanning microscopy.
目的对应用激光扫描共聚焦显微镜进行荧光强度的测定条件做了详细的分析。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
In most of cases, the laser scanning confocal microscopy images, such as fluorescence stained biomedical nuclei, are blurred and have a wide range of brightness and poor intensity homogeneity.
共焦显微图象如荧光染色细胞核图象，往往是一种比较模糊的图象，并且亮度范围大，灰度均匀度也较差。
Methods Fluorescence staining and confocal microscopy were used to study the distribution of contractile proteins in LAM cell.
方法用荧光抗体染色法和激光共焦显微镜观察收缩蛋白在L AM细胞的分布。
Methods Immunofluorescence technique was used to investigate whether there was HPK1 in hippocampus neuron under the fluorescence microscope and the confocal microscopy.
方法采用细胞免疫荧光技术通过免疫荧光显微镜和激光共聚焦显微镜观察HPK1是否在大鼠脑细胞中存在；
The research was engaged in the effect of HMC toxin on microfilaments of N and C cytoplasmic male-sterile maize by fluorescence technique and confocal scanning microscopy.
本研究还利用荧光标记和激光共聚焦显微镜相结合的方法，研究了HMC毒素对同核异质体玉米根冠细胞微丝骨架分布的影响。
Laser-scanning confocal microscopy was used to monitor the distribution of green fluorescence.
激光共聚焦荧光显微镜观察活细胞内荧光布局。
A laser scanning confocal microscopy (LSCM) and a fluorescence flow cytometric methods (FFCM) were used to identify the effect of curcumin on the expression of LDL-R in the human lymphocytes.
以荧光试剂标记配体法，利用流式细胞仪技术和激光扫描共聚焦显微镜技术研究姜黄素对人淋巴细胞LDL - R表达的影响。
Through many years" hard work, we successfully developed the first commercial Laser Confocal Scanning Fluorescence Microscopy and the first Gene- chip Scanner in our country."
通过多年的努力奋斗，成功地开发出国内首台商品化的激光共聚焦扫描荧光显微镜，并且成功开发出国内首台基因芯片扫描仪。
Through many years" hard work, we successfully developed the first commercial Laser Confocal Scanning Fluorescence Microscopy and the first Gene- chip Scanner in our country."
通过多年的努力奋斗，成功地开发出国内首台商品化的激光共聚焦扫描荧光显微镜，并且成功开发出国内首台基因芯片扫描仪。
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