Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳(2DPAGE)与质谱(MS)是分辨和鉴定蛋白的最常用的方法,但也存在有缺点。
The protein composition of sericin have been studied by polyacrylamide gel electrophoresis.
用聚丙烯酰胺凝胶电泳法研究丝胶的蛋白质组成问题。
Objective to establish the normal reference values of serum protein with agarose gel electrophoresis (AGE) in local clinical laboratory.
目的制定本实验室琼脂糖凝胶电泳法(age)测定血清蛋白的正常参考值范围。
The qualitative patterns of protein synthesis in nuclear transplant rabbit embryos were examined by SDS-polyacrylamide gel electrophoresis followed by silver staining.
采用SDS-聚丙烯酰胺凝胶电泳及银染法,对兔核移植胚胎蛋白质合成类型的变化进行了研究。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
The difference of this chapter is that we use an optimized one-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry combined protein mixture identification technology.
所不同的是我们采用了优化的一维凝胶电泳和液相色谱串联质谱联合的复杂蛋白质混合物鉴定技术。
The experimental procedures of agarose gel electrophoresis for detecting urinary protein were improved, and the LDH isoenzymes and lipoproteins could be analysed simultaneously.
本文对尿蛋白琼脂电泳法进行了改进,可以与L DH同二酶、脂蛋白同步电泳。
The electrophoertograms of protein and alkaline phosphatase isoenzymse. of serum in black pig were analysed by disc polyacrylamide gel electrophoresis method.
用聚丙烯酰胺凝胶圆盘电泳分析了黑猪血清蛋白质和碱性磷酸酶同工酶生化位点多态性的分布。
Two methods, high performance liquid chromatography and gel electrophoresis (SDS-PAGE), were used to evaluate the influence of different dyes'structures on protein labeling.
同时应用高效液相色谱和聚丙烯酰胺凝胶电泳(SDS-PAGE)两种分析技术研究染料结构对蛋白质标记的影响。
Method: Gel electrophoresis of soluble protein.
方法:可溶性蛋白质凝胶电泳。
The result of polyacrylamide gel electrophoresis shows that the protein band of expression is found at the position of about 55kd.
聚丙烯酰胺凝胶电泳实验结果表明在大约55kd的位置上有表达的蛋白质条带。
The SDS gel electrophoresis analysis shows that the expression of EPSPS recombined protein is over 55% and the purified EPSPS recombined protein reached as 90%.
SDS电泳分析结果表明EPSPS重组蛋白的表达量在55%以上,纯化的EPSPS重组蛋白纯度可以达到90%。
Method Gel electrophoresis of soluble protein was used.
方法可溶性蛋白质凝胶电泳。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
目的探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
目的探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
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