AMP was obtained by sephadex gel filtration.
提取的粗提物经凝胶过滤后得到抗菌肽。
METHODS: Gel filtration chromatography was adopted.
方法:采用凝胶色谱法。
Polyacrylamide gel filtration was used as the method for comparison.
以聚丙烯酰胺凝胶过滤作为参比方法。
Purpose To study the effects of different elements on protein separation during gel filtration.
目的研究凝胶过滤各影响因素对蛋白质分离的影响。
We extracted and purified osteocalcin(BGP) from calf femur by gel filtration, FPLC and HPLC subsequently.
本研究用凝胶过滤,快速液相(FPLC)和高压液相(HPLC)成功地从小牛股骨提取和纯化了骨钙素(BGP)。
Method The acupuncture serum were separated by gel filtration with Sephadex G 75 and molecular standards.
方法采用凝胶过滤层析技术对针刺全血清进行分段分离,并将分离的血清经冷冻干燥制成干粉。
Objective to find out the optimal condition for separation of the protein mixture by gel filtration chromatography.
目的探讨凝胶过滤层析分离蛋白质的最佳实验效果。
Pure enzyme was obtained by using the salt fractionation with ammonium sulphate, the ion-exchange chromatography and the gel filtration.
使用硫酸铵分级盐析,离交色谱,凝胶过滤可获得纯净的酶。
Selenium-containing phycocyanin(Se-PC)was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography.
使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白。
The changes of the molecular weight distributions of xylan during enzymatic hydrolysis have been investigated with gel filtration chromatography.
采用凝胶过滤色谱法研究了木聚糖在酶解过程中分子量分布的变化。
Biliary protein was isolated and purified by ammonium sulfate fraction and Sephadex G-75 gel filtration column chromatograph from complex pig bile.
采用了凝胶过滤结合硫酸铵沉淀法从异常复杂的胆汁中成功分离出一种蛋白质。
Methods: The gel filtration chromatography with Sephadex G-25 was used to separate different components from the small intestine extracts of sheep.
方法:葡聚糖凝胶分离及反相高效液相色谱法提纯 羊小肠活性抗菌肽;
The method of batch ion exchange chromatography combined with gel filtration chromatography to purify Human-like Collagen Biopolymer I was investigated.
研究了利用批量离子交换层析与凝胶过滤层析相结合的方法纯化类人胶原蛋白i的最佳条件。
Two major allergens PT 13 and PT 53 in platanus hispanica pollen were isolated and purified by ion exchange chromatography and gel filtration chromatography.
以离子交换和凝胶过滤层析对梧桐花粉变应原进行分离和纯化。
Compared with gel filtration standard showed that the relative molecular weight of POD is about 45kd as well, which indicate that the purified POD is monomer.
分子筛分子量测定结果显示整蛋白分子约为45kd左右,表明分离得到的POD为单体蛋白,这与之前的研究结果有很大的不同。
This article lists the polyacrylamide gel electrophoresis(PAGE), high-performance liquid chromatography(HPLC), gel filtration chromatography, methods combined.
列出了聚丙烯酰胺凝胶电泳(PAGE)、高效液相色谱(HPLC)、凝胶过滤色谱、方法联用等分离低分子量肽的方法。
Contents of MMC and magnetic fluid in the magnetic pro-drug were measured by gel filtration chromatography and atomic absorption spectrophotometry, respectively.
产物经葡聚糖凝胶柱分离紫外分光光度法和原子吸收分光光度法分别测定其丝裂霉素含量和磁流体含量。
The vacuolating Cytotoxin from Helicobacter pylori YC-11 was purified by using water-extracting, salt precipitation and gel filtration chromatographic fractionation.
运用水油提、盐析及凝胶过滤色谱法对幽门螺杆菌YC-11株所分泌的形成空泡细胞毒素进行了提取。
Methods Gel filtration chromatography and cadmium-injured Wistar rats were used to observe the action of nonprotein bound cadmium and total cadmium in renal lipid peroxidation.
方法利用染镉动物和凝胶渗透层析技术,观察非蛋白结合镉、总镉在肾脂质过氧化时的作用。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
The water-soluble, urea-soluble and intrinsic membrane proteins ( WSP, USP and MIP ) from human clear, cortical punctate opaque and senile cataract lenses were studied by gel filtration and SDS-PAGE .
对老年透明晶状体和老年性白内障晶状体水溶性蛋白(WSP)、尿素溶蛋白(USP)和纤维细胞膜内在蛋白进行了分析。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
菌体经过超声破碎后反复洗涤包涵体,融合蛋白纯度达到70%,进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The products obtained were purified by extraction with copper sulfate solution, filtration through silica gel, recrystallization and vacuum sublimation.
粗产物经硫酸铜溶液萃取、硅胶过滤、重结晶、真空升华进行分离提纯得到纯产品。
Methods: MMA was prepared with asialo-fetuin affinity and gel-filtration chromatography form murine melanoma.
方法:利用亲合层析及凝胶过滤层析从小鼠黑色素瘤中提取小鼠黑色素瘤凝集素(MMA)。
This study is not only a breakthrough the bottleneck of silica gel formation and pulp filtration, but also a good removal iron and silicon completely, and a filterable cake is obtained.
本研究突破了在高硅铜冶渣湿法浸出回收有价金属过程中容易形成硅胶所造成难过滤的瓶颈;
The experiment results showed that preformed weak gel had many characteristics such as shear dilute rheological characteristic, anti-shear, easy injection and low filtration;
实验结果表明,一次交联聚合物弱凝胶具有剪切稀释的流变特征,耐剪切,注入性好,滤失量低;
The results showed that the methods of isoelectric precipitation, gel-filtration chromatography and anion-exchange chromatography were suitable for purifying the major allergens in buffalo milk.
实验结果表明,等电点沉淀、凝胶柱层析法和阴离子交换法适合于分离水牛乳中主要过敏原成分。
Methods DEAE-cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides whose structures were characterized by using chemical and spectral methods.
方法采用DEAE -纤维素柱色谱和凝胶渗透色谱进行分离纯化,化学和光谱方法分析其结构特征。
Methods DEAE-Cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides, whose structures were characterized by chemical and spectral methods.
方法采用DEAE -纤维素柱色谱和凝胶渗透色谱分离纯化,化学和光谱方法分析其结构特征。
Systematically evaluate its behavior, including: foundation liquid characteristic, rheological characteristic, gel break characteristic, filtration property and clean-up property.
并且对压裂液体系的性能进行了评价,包括泡沫压裂液基本参数、耐温耐剪切性能、流变性能、破胶性能、滤失性能等。
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