Hantaan virus; glycoprotein; gene cloning.
汉滩病毒；糖蛋白；基因克隆。
Objective: To develop the gene cloning of NGF from rat brain.
目的：克隆大鼠脑组织中神经生长因子基因。
Transposon tagging is an effective method for plant gene cloning and isolation.
转座子标签法是克隆与分离植物基因的一项十分有效的方法。
In recent years, some studies about gene cloning of this enzyme have got much achievement.
近几年猪肝酯酶的基因克隆研究也取得了较大进展。
Specific primer PCR was a gene cloning technique with high efficiency, stability and accuracy.
特异引物PCR方法是一种高效、稳定、可靠的基因克隆手段。
So far, only Bacillus subtilis 168 and its mutants have been used for gene cloning in the species.
迄今文献中报道枯草杆菌基因克隆化都采用枯草杆菌168株及其突变体。
The facility functions as a training center for scientists in gene cloning and protein production.
设备为在基因过程中的科学家克隆和蛋白质生产起一个培养训练中心的作用。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
Purpose: AQP4 M23 gene cloning and construction in the expression vector can provide us a research tool.
目的克隆大鼠水通道蛋白4（AQP4）M23基因并构建其载体为进一步研究提供理论基础和研究工具。
As main edible oil, researches on biosynthesis pathway and relative gene cloning have become hot research topics.
植物油脂作为主要的食用油，其生物合成途径及相关基因的克隆已成为研究热点。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
This paper reviews the current achievement in the field of gene cloning and function studies of plant cellulose synthase.
本文综述了近年植物纤维素合酶及其相似蛋白编码基因的研究进展。
Conclusions: Bioinformatics analysis provided evidences for PPIL6 gene cloning and further study of the functions of PPIL6 gene.
结论：生物信息学分析为进一步进行PPIL6基因的克隆及功能研究奠定了基础。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
Moreover, saturated genetic maps consisting of molecular markers can be used as a reference for gene mapping, gene cloning and maker-assisted selection (MAS) in Capsicum.
而饱和的分子标记连锁遗传图谱是辣椒基因定位、克隆和分子标记辅助育种的重要工具。
In this paper, the lysozyme on its structure, separation and purity, mechanism, gene cloning were reviewed. Medicinal application and developing foreground of lysozyme was also discussed.
本文综述了溶菌酶的结构特点、作用机制、分离纯化、基因克隆等方面的研究现状，并对其医药应用和发展前景做了展望。
SRAP and TRAP are being applied in germplasm genetic diversity analysis, genetic map including transcriptome map construction, important trait gene tagging and gene cloning in many crops.
目前这两种标记系统已开始在多种作物的种质资源鉴定评价、遗传图谱构建(包括转录图谱)、重要性状标记乃至基因分离克隆等方面成功应用。
To look for a good method to producing NGF which has low molecular weight, we designed two projects which are carried out through gene cloning and cell engineering techniques to provide the problem.
为了能寻求一种切实可行及表达高活性小分子ngf的生产途径，我们通过基因工程和细胞工程技术设计了两种NGF的体外重组方案。
So when scientists say they are cloning the tree's gene, he believes they are really propagating a holy spirit.
因此当科学家宣称他们正在克隆这棵树的基因的时候，他认为他们实际上是在繁殖一个神圣的灵魂。
Besides the low efficiency of cloning - just 1.7 per cent of embryos came to term - another challenge to creating transgenic dogs is controlling where in the nuclear DNA a foreign gene lands.
除了低克隆率-只有1.7%的胚胎能存活到最后阶段-创造转基因狗还有一个挑战即如何控制外部基因在核d NA中的植入位置。
The successful cloning of monkey HCBP6 gene paves a way for the study of the relationship of structure and function of HCBP6 genes from different species.
猴hcbp6同源基因的克隆化为研究不同种属来源的HCBP6基因的结构域功能、表达与调控等研究奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
This shows that a xylose isomerase gene was missing in the yeast, and cloning of this gene from other organisms might make the yeast ferment xylose directly.
这表明，它缺少异构酶基因，如果将其它微生物的这种基因克隆到酵母中去，它就能直接发酵木糖。
Cloning of plant resistance gene is greatly helpful to crop resistance breeding and the insight of resistance mechanism.
植物抗病基因克隆研究对于抗病育种和抗病机制的理解具有重要意义。
All in all, it is responsible to apply this kind of positional cloning strategy for finding the disease gene in this big family.
总之，本研究所采用的定位方法对于这个家系的致病基因的准确定位是合理的。
Objective Cloning and screening a novel candidate gene related to developing mouse cleft palate.
目的克隆并筛选与小鼠腭裂发生相关候选新基因。
It is a basic work for the application of marker-assisted selection and cloning of anti-disease gene in Japanese flounder selective breeding program.
这些抗病性候选标记的获得为实现牙鲆分子标记辅助育种和抗病基因克隆奠定了一定基础。
It is a basic work for the application of marker-assisted selection and cloning of anti-disease gene in Japanese flounder selective breeding program.
这些抗病性候选标记的获得为实现牙鲆分子标记辅助育种和抗病基因克隆奠定了一定基础。
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