It led to a new technology, Gene Targeting.
这也引出一项新的技术——基因靶向技术。
Conclusion The mouse EMSP1 gene targeting vector is constructed successfully.
结论：成功构建小鼠emsp1基因打靶载体。
And immunological regulators and gene targeting treatment are new ways for treatment.
另外免疫调节、基因靶向治疗为其提供了新的治疗思路。
Gene targeting technology, has brought the possibility of human transformation of life.
基因靶向技术的进步，带来了人类改造生命的可能。
Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine (6, 7).
基因靶标研究显示t CF 4是维持小肠的隐窝干细胞所必需的(6,7)。
Gene targeting technology and its application in research of animal model of human disease were briefed in this paper.
本文就基因打靶技术及其在人类疾病动物模型研究中的应用做一介绍。
Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation.
基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定点修饰的实验方法。
Briefly introducing concepts and the primary technical procedure of gene targeting, and systematically introducing selective methods and gene targeting strategies.
简要阐述了基因打靶的概念和基本环节，系统介绍了筛选方法和基因打靶的策略。
Briefly illustrating concepts and the primary technical procedure of gene targeting, and systematically introducing selective methods and gene targeting strategies.
简要阐述了基因打靶的概念和基本环节，系统介绍了筛选方法和基因打靶的策略。
These knockout procedures are now commonly done in mice and rats, and three researchers earned a Nobel prize in 2007 for developing this method, called "gene targeting".
这种基因敲除程序现在已经被普遍用于小鼠和大鼠，3名研究人员因为发明了这种“基因打靶”技术获得了2007年的诺贝尔奖。
Gene targeting technology has been widely applied to mouse es cell, but more difficult to livestock species because es cell of livestock species could not differentiate into germ cell line.
基因打靶技术在小鼠es细胞上被广泛应用，但由于大家畜很难制备向生殖系分化的ES细胞系，所以基因打靶的应用变得非常困难。
These results demonstrate the utility of the gene targeting approach in the study to investigate the gene function of T. gondii. and the mechanism by which this parasite invades to the host cells.
结论通过构建基因打靶载体 ，可有目的地敲除弓形虫膜抗原基因 ，为进一步研究弓形虫侵入机制 ，探讨弓形虫病防治提供可行的方法。
There are other examples of success in specifically targeting genetic changes in never-smokers with lung cancer, such as treatments of tumors with alterations in the EML4-ALK gene.
针对不吸烟者的基因改变进行靶向治疗，这有些其它的成功例子，比如针对有EML4-ALK基因改变的肿瘤进行的治疗。
However, this ruling is merely the beginning of a lengthy legal battle, with similar suits likely to pop up around the country targeting other gene patents, and with an appeal in the works.
然而，这只不过是一场漫长的，包括突然出现的指向其他基因专利的小型诉讼，及其自身上诉审在内的法律战争前哨战而已。
In recent years the research on targeting in gene therapy has made much exciting progress.
基因治疗的靶向性研究近年来取得了许多新的进展。
Conclusion Targeting mutation of Fox C2 gene can result in ventricular septal defect.
结论：敲除叉头框c 2基因可使小鼠室间隔发育缺损。
Nano-delivery system increases cellular uptake of specific gene, protects them against nucleases and enhances their targeting and selectivity.
纳米控释系统使转染基因的胞内摄入量增高，增强其稳定性、靶向性及细胞定位。
A major goal of tumor-targeting gene therapy is to transfer genes efficiently to tumor cells by targeted vectors.
肿瘤靶向基因治疗的主要目的是用靶向性的载体将治疗基因靶向传递到肿瘤细胞。
Yet, researchers still have a long way to go in order to reach the goal of applying the targeting gene therapy in clinical practice.
可是要实现靶向基因治疗应用于临床实践的目标，还需要基础研究的进一步深入。
Objective To evaluate the possibility of hepatitis B virus (HBV) as a vector in liver-targeting gene therapy.
目的探讨乙型肝炎病毒（HBV）作为肝靶向性基因治疗载体的可能性。
Objective: to construct and identify a lentiviral vector harboring RNAi sequence targeting the human high mobility group A1 (HMGA1) gene.
目的：构建和鉴定人高迁移率族蛋白组a 1 (HMGA1)基因rna干扰慢病毒表达载体。
Objective to modulate the molecular conformation of the hepatocyte targeting drug to increase exogenous gene expression efficiency in the targeted cell.
目的：用调整肝细胞靶向基因药物分子构形的方法，以期提高外源转入基因在目标细胞的表达效率。
AIM: to construct a recombinant adenovirus vector carrying AFP promoter to specifically express a targeting gene in hepatocellular carcinoma HepG2 cells.
目的：建立一种在甲胎蛋白(afp)阳性的肝癌细胞中靶向表达目的基因的重组腺病毒载体。
As an important gene transfer vector, adenovirus has the deficiency of poor targeting.
腺病毒作为一种重要的基因转移载体，在应用中存在着靶向性差的问题。
Objective: to be as the foundation of the tumor therapy with targeting IL-21 gene by constructing of recombinant interleukin 21 gene adenovirus expression vector.
目的：构建含人IL -21基因的重组腺病毒表达载体，为IL - 21基因治疗肿瘤研究奠定基础。
This paper introduced the principle, the targeting strategies, the screening mechanism and the commonly method in animals, plants and micro-organisms of gene knockout.
本文简述了基因敲除技术的基本原理、打靶策略、筛选机制，在动植物和微生物中常用的基因敲除方法以及基因敲除的应用。
TK contained ALBTRS promoter sequence which probably control the targeting expression in hepatocyte of target gene TK. The enzyme cut assay of the vector LN. ALBTRS.
TK的结构中，含有ALBTRS启动子，具有在表达白蛋白的肝细胞中特异表达的潜能，载体经酶切鉴定表明结构符合要求。
However, gene therapy for cancer is also faced with a specificity problem: the specific targeting of transgene expression to the site of the tumour.
然而，肿瘤基因治疗同样面临一个特殊问题即转基因特异性表达于肿瘤部位问题，靶向性基因转导依然是目前基因治疗的一大障碍。
However, gene therapy for cancer is also faced with a specificity problem: the specific targeting of transgene expression to the site of the tumour.
然而，肿瘤基因治疗同样面临一个特殊问题即转基因特异性表达于肿瘤部位问题，靶向性基因转导依然是目前基因治疗的一大障碍。
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