At last, we got one positive hybridoma cells named 4d11.
筛选共得到一株阳性杂交瘤细胞，命名为4d11。
This is a reliable method to select the hybridoma cells for McAb of IBDV.
是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。
Then the hybridoma cell lines secreting high titer of McAbs were screened.
目的建立分泌抗华支睾吸虫代谢抗原的单克隆抗体杂交瘤细胞株。
The chromosome of the positive hybridoma and the character of the mAb were analysed.
对所得阳性杂交瘤细胞作染色体分析并对单克隆抗体进行特性分析。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.
通过试管法和玻片法检测抗a抗体的效价和亲和力。
We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.
用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。
The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.
杂交瘤技术原理和操作过程包含了其许多优点，特异性强、方便、敏感和快速。
Absorption of human-human hybridoma cells can abolish the human hybridoma growth factor (HGF) activity but not the BCGF activity.
但人-人B细胞杂交瘤可吸收掉上述BCGF活性上清中的促杂交瘤细胞生长因子(HGF)活性，而对BCGF活性无明显影响。
Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体，鼠源性抗体可变区基因的克隆与测序是关键。
Highly positive rate was obtained. The four hybridoma cells were separately cloned nine times in proper sequence and can continuously secrete high-titer McAbs.
它们分别经过九次有限稀释连续培养九个月仍能持续地分泌高滴度抗体，并且抗体分泌量随克隆化次数的增多而增加。
The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
将纯化之球蛋白片段免疫 BALB/c小鼠后， 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1进行细胞融合。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.
采用杂交瘤技术制备人源性单克隆抗体存在许多困难，90年代初期出现了抗体库技术，为抗体的制备提供了新的途径。
Since 1975 when the technology of hybridoma and monoclonal antibody was developed, many investigators have been paying close attention to antibody targeting therapy for patients with cancer.
自从1975年发现了单克隆抗体技术和不久后提出生物导弹设想以来，导向治疗受到许多科研工作者的关注。
Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.
研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。
Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.
研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。
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