Then the hybridoma cell lines secreting high titer of McAbs were screened.
目的建立分泌抗华支睾吸虫代谢抗原的单克隆抗体杂交瘤细胞株。
Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体，鼠源性抗体可变区基因的克隆与测序是关键。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
将纯化之球蛋白片段免疫 BALB/c小鼠后， 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1进行细胞融合。
The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
将纯化之球蛋白片段免疫 BALB/c小鼠后， 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1进行细胞融合。
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