METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
The present study investigated the methodology of synthesizing two single-staining immunofluorescence images into one overlapped double-stained image by using computerized image-processing techniques.
探讨以计算机图像处理技术将普通荧光显微镜采集的同视野单标记免疫荧光图像合成为双标记图像的方法。
Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment.
方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。
METHODS: Immunofluorescence histochemical double-staining method was used.
方法：免疫荧光组织化学双重标记技术。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
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