To improve the cell culture method of BTM cells in vitro.
探索并改进体外牛眼小梁细胞的培养方法。
ObjectiveTo establish a new method for purification culture of retinal ganglion cells in vitro.
目的建立一种简单的体外培养视网膜神经节细胞的方法。
METHOD: In vitro, the hemagglutination reaction of Feiduqing Granules were tested in the chicken embryo culture.
方法：体外抗病毒实验采用鸡胚法，以血凝反应为测定指标。
Methods We use the model of cortical neuronal culture in neonatal rats in vitro and observe the NSE, NGF and IGFR expressions in cultured neurons by immunohistochemical method.
方法采用体外新生大鼠皮层神经细胞培养法观察微量元素碘对神经细胞表达nse、NGF和IGFR蛋白的影响。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
The paper reported the effects of cerebrolysin and neuropeptide on cerebral neurocyte in newborn rat by vitro culture method, and measured the length of cellular process and the diameter of cells.
运用体外培养的新生鼠大脑神经细胞来观察脑活素及神经肽对其的影响，并测量突起长度及胞径，与对照组进行统计学处理。
AIM: to establish a stable method to isolate and culture human umbilical vein endothelial cells in vitro.
目的：建立稳定的人脐静脉内皮细胞分离和培养的方法。
Objective: to investigate the culture method in vitro and the bionomics of masticatory muscle satellite cells.
目的：探讨咀嚼肌卫星细胞的体外培养方法并研究其生物学特性。
Objective: to explore the primary culture method of human chorionic and decidual tissues in vitro and to study their growth regular.
前言：目的：探讨体外培养人绒毛膜组织和蜕膜组织的方法，并对其体外生长规律进行研究。
Objective: To establish a convenient and efficient method of primary culture of rabbit brain microvessel endothelial cells(BMVEC) in vitro.
前言： 目的：建立简便、有效的兔脑微血管内皮细胞体外原代培养方法。
The differences between the absorption of leucine and uracil by various parts of mid-gut of silkworm larva were studied, using trace method and culture in vitro technique. During in vitro.
应用示踪法和体外培养技术，研究了桑蚕幼虫中肠各部位对亮氨酸和尿嘧啶吸收作用的差异。
Objective: To establish a method of culture of retinal neuron cells in vitro.
目的：建立新生大鼠视网膜神经元体外培养的有效方法。
Methods Human fetal limbal epithelial tissue was cultured in vitro by enzymatic digestion culture method and the morphological characteristics of cultured cells were recorded.
方法应用消化培养法对人胎儿角膜缘上皮组织进行体外培养并观察记录培养细胞的生长特性；
Subculture is an important link in the process of the tissue culture and the long-term subculture is the essential method for conservation in vitro of germplasm.
在植物组织培养过程中，继代培养是其中的重要环节，而长期继代则是种质资源离体保存的必要手段。
The method using in vitro irradiation seems to be more effective than conventional irradiation, tissue culture or backcross for improving a few characters in a variety.
因此，对于品种个别性状的改造，离体辐照可能比其它方法更有效。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
The effects of genistein and daidzein on the proliferation of human breast cancer cell line in vitro were studied by cell culture and MTT colorimetric method.
应用体外细胞培养和MTT比色法。研究大豆异黄酮类化合物金雀黄素和大豆黄酮对人乳腺癌细胞系体外增殖作用的影响。
Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods.
方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
Objective: To establish a new method for culture of human spleen dendritic cells (DC) for being used in vitro study.
目的：建立人脾脏单核细胞体外培养生成大量树突状细胞的新方法。
Objective: To study the effective method of isolation, culture and proliferation of rat bone marrow-derived dendritic cells (DC) in vitro.
目的：探讨体外分离、培养和扩增大鼠骨髓来源树突状细胞(DC)的有效方法。
ObjectiveTo investigate rabbit's lens culture in vitro and its growth so as to find out a simple and easy operational method.
目的探讨兔晶状体培养及其体外生长情况，试图找到一种简便、易行的实验方法。
Objective:To explore the method of isolate and culture of human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro, and identify their biological characteristic.
目的：探讨体外分离培养人脐带间充质干细胞的方法，并对其进行生物学鉴定。
We studied the protective effect of HPQP (hydroxypiperaquine phosphate) on the alveolar macrophages of guinea pig in the cells culture in vitro by the modified Myrvik method.
本文用体外培养的方法，研究了HPQP对肺泡巨噬细胞的保护作用。
Methods the effects of TGA on oocyte maturation and in vitro fertilization (IVF) in mice were studied by the method in vitro culture and IVF in mice oocyte.
方法：采用小鼠卵母细胞体外培养、体外受精的方法研究了铅对小鼠卵母细胞的成熟和体外受精的影响。
To explore the culture method of rat dental follicle cells in vitro.
探讨大鼠牙囊细胞体外培养的方法。
Objective: the main idea of this study was to explore the method of the normal ameloblast culture in vitro.
目的：探索成釉细胞离体培养方法，建立成釉细胞体外模型。
To improve the method of myoblast cell culture in vitro.
改进体外成肌细胞培养方法。
Objective To study different methods of in vitro culture of human epithelial ovarian carcinoma, and the method of inducing drug-resistance cell line it.
目的研究人卵巢上皮癌体外培养的方法及其耐药株的诱导方法。
Method: The method of cell and tissue culture was utilized to carry out an observation on the cytopathy effect(CPE) led by influenza virus FM1 with the aerosol of different concentrations in vitro.
方法：体外试验采用组织细胞培养法，观察不同浓度药物对流感病毒FM1病毒株致细胞病变（CPE）的作用；
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