Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography.
方法：采用离子交换柱层析和凝胶柱层析纯化得到RRTP；
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