The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The induction temperature, induction time, and IPTG concentration were also optimized by a series of experiments. Further purification modes of this protein were also explored.
同时还进行梯度实验分别对诱导的温度、时间和IPTG诱导时菌体浓度进行优化，并对蛋白的纯化方案进行摸索。
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。
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