After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.
用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35；
Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.
用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35；
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