Evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
探讨应用活细胞荧光染料 CFSE 检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Methods:To observe the effects of the Chitooligosaccharidean and GAH on the lymphocyte proliferation of mouse by MTT assay and the phagocytosis of macrophages by Tuluylene red pinocyfic test.
方法：应用MTT法观察体外壳寡糖和GAH对小鼠脾淋巴细胞细胞增殖的影响，应用巨噬细胞吞噬中性红试验观察壳寡糖和GAH对小鼠腹腔巨噬细胞吞噬功能的影响。
Methods:To observe the effects of the Chitooligosaccharidean and GAH on the lymphocyte proliferation of mouse by MTT assay and the phagocytosis of macrophages by Tuluylene red pinocyfic test.
方法：应用MTT法观察体外壳寡糖和GAH对小鼠脾淋巴细胞细胞增殖的影响，应用巨噬细胞吞噬中性红试验观察壳寡糖和GAH对小鼠腹腔巨噬细胞吞噬功能的影响。
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