Methods Phosphoproteins were extracted from lysates of normal mice livers by phosphate metal affinity chromatography (PMAC) resin.
方法取正常小鼠肝脏,裂解肝脏后利用磷酸盐金属亲和层析(PMAC)树脂提取磷酸化蛋白。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化,其纯度可达91%。
Immobilized metal affinity chromatography (IMAC) is a well-established protein separation technique experiencing expanded use based on new, high-performance analytical phases.
固定化金属离子亲和色谱(IMAC)作为一种新型高效色谱分离模式,已经被广泛应用于蛋白质的分离分析。
Cu(II)-immobilized metal affinity chromatography (Cu(II)-IMAC), as a highly reliable analytical procedure, has been widely used in separation and purification of biomacromolecule.
铜离子固定金属亲和色谱作为一种有效的分析方法,已普遍应用于生物大分子的分离与纯化。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
包涵体蛋白经含8m尿素的结合缓冲液溶解后,用金属亲和层析法进行纯化,获得了高纯度的目的蛋白。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
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