The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
组织块培养法对于原代细胞的培养要优于酶消化法。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
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