The effect of the mobile phase composition on the separation of the components was discussed.
探讨了流动相组成对原料， 溶剂和产物分离的影响；
The effects of mobile phase composition, flow rate, column temperature, gradient ramp, ph value, salt concentration, and buffer concentration on capacity factors (K ') were respectively discussed.
对流动相组成、流率、柱温、梯度速率、pH值、盐浓度以及缓冲液浓度对容量因子(K')的影响分别进行了讨论。
Methods Under linear scale up mode, overlapping separation range map was used to optimize the composition of mobile phase.
方法在线性放大模式下，使用重叠分辨图法对流动相组成进行优化调节。
METHODS: Fixing other conditions than the composition and ratio of the mobile phase, estimate the optimum according to the separation results.
方法：固定其他色谱条件，改变流动相组成与配比，以分离结果的优劣优化流动相。
The influences of the composition of mobile phase, the flow rate, the temperature and the injected quantity on the enantiomer rententivity and selectivity were examined and discussed.
考察了流动相组成、流速、进样量和温度等因素对对映体分离的影响。
Experimental results further showed that dead volumes were also related to factors, such as the characteristics of the mobile phase, its composition and the column temperature.
结果表明死体积与流动相种类、配比和柱温等因素有关。
The composition of mobile phase has different influence on the elution range and ability to the two types of solutes.
流动相组成对这两种类型溶质的洗脱范围及洗脱能力的影响不同。
The effects of temperature, flow rate and composition of mobile phase on retention, resolution, column efficiency and the pressure of the system were examined.
考察了温度、流速和流动相组成对酚类样品的保留、分辨、柱效和系统压力的影响，探讨了酚类样品在聚合物柱上的热力学行为。
The effects of composition of mobile phase, the structure of solute and the column temperature on chiral separation were extensively studied. The chiral recognition mechanism was discussed.
考察了流动相组成、溶质结构和柱温对对映体分离的影响，并初步探讨了手性识别的机理。
The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
The composition of mobile phase, flow rate and column temperature were optimized to get better resolution.
考察了流动相组成、流速和柱温对分离度的影响。
The composition of mobile phase, flow rate and column temperature were optimized to get better resolution.
考察了流动相组成、流速和柱温对分离度的影响。
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