Molecular cloning and sequence analysis were performed.
并进行分子克隆和测序分析。
Purpose Molecular cloning and expression of pro nattokinase gene with e.
目的构建纳豆激酶原基因克隆，实现其在大肠杆菌中的表达。
Objective Molecular cloning and sequencing of the human nerve growth factor gene.
目的克隆人神经生长因子基因编码区。
These results provide a basis for molecular cloning and production application of DS1.
本研究为该基因的克隆及其在生产中的应用奠定了基础。
For this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work.
本论文基于此目的，开展了青蒿素生物合成相关基因的分子克隆工作。
Technology of molecular cloning was employed to construct standard allelic ladder of DYS643 locus and used for DYS643 genotyping.
并通过分子克隆技术制备dys643基因座的等位基因分型标准物。
The high molecular weight glutenin subunits and their encoding genes in Aegilops variables were characterized by SDS-PAGE and molecular cloning.
利用SDS-PAGE和分子克隆方法研究了易变山羊草的高分子量谷蛋白亚基及其基因。
Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
方法采用基因克隆、测序及生物信息学技术，分析HLA新等位基因与HLA已知基因序列的差异。
These will provide abundant and reliable molecular markers for gene location and molecular cloning in the coming genetic engineering breeding of the strains.
并将为下一步的基因工程育种工作中的基因定位和分子克隆提供丰富可靠的分子标记。
But NBAC members are planning to word the recommendation narrowly to avoid new restrictions on research that involves the cloning of human DNA or cells-routine in molecular biology.
但是，NBAC成员们计划在建议的措辞上更为严谨，以避免给克隆人体DNA或细胞等研究带来更多地限制——因为这是分子生物研究中的常规课题。
(Also known as genetic engineering, molecular biology technology or cloning.) the collection of techniques that allow the purification, manipulation and use of genetic material.
(也称基因工程、分子生物学技术或克隆)纯化、处理和使用遗传材料的技术总和。
But NBAC members are planning to word the recommendation narrowly to avoid new restrictions on research that involves the cloning of human DNA or cells - routine in molecular biology.
但是，NBAC成员们正计划在建议的措辞上更为严谨，以避免给克隆人体DNA或细胞等研究带来更多地限制——(这属于)分子生物研究中的常规课题。
DNA, complementary; cloning, molecular; sequence analysis; gene expression.
互补；克隆分子；序列分析；基因表达。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
Genetic linkage map is a QTL mapping, the genetic map Spaces cloning, comparative genomics research and and molecular marker-assisted breeding the foundation.
遗传图谱是数量性状基因定位(QTL)、基因图位克隆、比较基因组学研究以及分子标记辅助育种等的基础。
Methods Molecular biology technologies of RT-PCR, directed cloning and DNA sequencing were carried out.
方法采用RT -PCR、定向克隆和DNA测序等一系列分子生物学技术进行本实验。
Recent advances in cloning of avirulent genes in pathogens and resistant genes in plants, together with molecular mechanism of plant disease resistance, were reviewed.
报告了病原无毒基因和植物抗病基因克隆；植物抗病分子机制的研究进展。
Most of the research about blast resistance focuses on molecular marker and mapping and cloning of blast resistance (related) genes.
目前对水稻抗瘟性的研究主要集中在抗瘟性基因的分子标记和抗瘟性(相关)基因的克隆两方面。
This work has provided a solid basis for molecular marker_assisted selection (MAS) for disease resistance, and made location and cloning of disease resistant genes possible.
这为进行抗病分子标记辅助选择，以及最终定位与克隆其抗病基因打下了良好基础。
To understand the molecular basis of this QTL, we isolated the SKC1 gene by map-based cloning and found that it encoded a member of HKT-type transporters.
为了研究该QTL的分子基础，我们通过图位克隆的方法分离到了SKC1基因，发现它编码一个HKT型转运蛋白。
The results supplied useful information for the QTL cloning and molecular breeding of rice grain shape.
这些结果为粒形qtl的克隆和分子育种奠定了基础。
Cloning, molecular --- Transfer of a DNA sequence into a single cell of a microorganism, followed by culture of the microorganism to produce large quantities of the DNA sample for analysis.
将DNA序列转移到微生物单细胞中，然后培养微生物，以产生大量DNA样本用以分析。
This is because molecular mechanism underlying animal cloning is not well known.
问题的根源在于对克隆基础的分子机理缺乏了解。
Moreover, saturated genetic maps consisting of molecular markers can be used as a reference for gene mapping, gene cloning and maker-assisted selection (MAS) in Capsicum.
而饱和的分子标记连锁遗传图谱是辣椒基因定位、克隆和分子标记辅助育种的重要工具。
In 1990s, molecular biological approach has led to a series of cloning and functional expression of ligand-gated P2X receptors and G-protein coupled P2Y receptors.
90年代，采用分子生物学手段，一系列配体门控的P2 X受体及G蛋白耦联的P 2y受体被克隆及功能表达。
Here we presented the overview of plant CMS fertility restorer gene mapping, cloning and its molecular mechanism. It was also discussed on utilization of fertility r...
文章综述了植物恢复基因的定位、克隆以及育性恢复分子机理的研究进展，并讨论了恢复基因在植物分子育种上的应用。
To look for a good method to producing NGF which has low molecular weight, we designed two projects which are carried out through gene cloning and cell engineering techniques to provide the problem.
为了能寻求一种切实可行及表达高活性小分子ngf的生产途径，我们通过基因工程和细胞工程技术设计了两种NGF的体外重组方案。
To look for a good method to producing NGF which has low molecular weight, we designed two projects which are carried out through gene cloning and cell engineering techniques to provide the problem.
为了能寻求一种切实可行及表达高活性小分子ngf的生产途径，我们通过基因工程和细胞工程技术设计了两种NGF的体外重组方案。
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