Cell viability was determined by MTT assay, cell cycle and apoptosis rate were analyzed by flow cytometer.
利用MTT法测定细胞成活率，流式细胞仪测试样品的细胞周期与凋亡率。
Cell viability was examined by methyl thiazolyl tetrazolium (MTT) assay and 50% inhibitive concentration (IC50) of cisplatin was examined also.
四甲基偶氮唑蓝比色法检测细胞存活率并计算顺铂的50%抑制浓度(IC50)。
Cell viability, the change of membrane fluidity, LDH activity in bath and lipid peroxidation injury were investigated by MTT assay, fluorometric technique and spectrophotometric method, respectively.
通过MTT法测定细胞存活率、分光光度法测定细胞LDH漏出率及细胞脂质过氧化水平，采用荧光标记技术研究细胞膜流动性变化。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
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