The objective is to observe the expression of MMP-1and TIMP-1 in nucleus pulposus cells of intervertebral disc degeneration.
目的是测定 MMP-1及TIMP-1在人退变椎间盘髓核中的表达。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
目的通过对人退变髓核细胞的原代培养,了解其生物学特性,并将绿色荧光蛋白基因转染至细胞内。
FGF-2 has been shown to retain the differentiation potential of monolayer expanded chondrocytic cells. However, its effect on nucleus pulposus cells is not known.
在单层培养的软骨细胞扩展中,FGF-2显示出可以保持分化的潜力,但此种作用在髓核细胞中尚不明确。
Direct contact of MSCs and nucleus pulposus cell in vitro is essential for BMSCs reversing the nucleus pulposus cells degeneration and differentiating into nucleus pulposus-like cells.
提示通过体外直接接触共培养,骨髓间充质干细胞能逆转髓核细胞退变,而且髓核细胞能诱导骨髓间充质干细胞向类髓核细胞转换。
Direct contact of MSCs and nucleus pulposus cell in vitro is essential for BMSCs reversing the nucleus pulposus cells degeneration and differentiating into nucleus pulposus-like cells.
提示通过体外直接接触共培养,骨髓间充质干细胞能逆转髓核细胞退变,而且髓核细胞能诱导骨髓间充质干细胞向类髓核细胞转换。
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