Express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
Objective To express severe acute respiratory syndrome(SARS) virus S2 protein in Pichia pastoris.
目的研究用毕赤酵母菌表达SARS病毒S2蛋白亚单位。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
Objective:To express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
目的：利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
In this paper, the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed.
本文实现了重组别藻蓝蛋白基因在毕赤酵母中的异源表达。
Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes.
结论毕赤酵母系统重组表达的膜联蛋白V具有较高的结合外露PS红细胞的能力。
The gene has homology as high as 60 percent with saccharomyces cerevisia GPD1 gene and 70 percent with Angus pichia yeast GPD gene.
其与酿酒酵母gpd 1基因同源性高达60%，与安格斯毕赤酵母gpd基因同源性高达70%。
Results By disrupting the PEP4 gene in Pichia pastoris, the productivity of heterologous protein expression increased 9.5% on average.
结果剔除毕赤酵母中的PEP4基因后外源蛋白质表达量平均提高9．5%。
Displayed on the pichia surface, CALB not only achieved self-immobilization, also has it's heat resistance and catalytic activity improved.
将其展示于毕赤酵母表面展示，不但实现了自固定化，耐热性和催化活性也有改善。
Conclusion Highly-active recombinant mutant human annexin V with endogenous metal-chelating sites can be expressed in Pichia Pastoris system.
结论 利用毕赤酵母系统表达出高活性的、具有内在金属螯合位点的突变型人膜联蛋白V。
Objective:To investigate the effects of different secretion signals on efficiency of onconase(ONC) secretion in Pichia pastoris expression system.
目的：研究不同分泌信号对豹蛙酶（ONC）在巴斯德毕赤酵母中分泌效率的影响。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
Pichia surface display technology has advantages in presents a variety of functional proteins, which has been made great study progress home and abroad.
其中利用毕赤酵母表面展示技术展示各种功能蛋白具有更大的优越性，在国内外研究中都取得了较大进展。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
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