Express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
Objective To express severe acute respiratory syndrome(SARS) virus S2 protein in Pichia pastoris.
目的研究用毕赤酵母菌表达SARS病毒S2蛋白亚单位。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
Objective:To express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
目的：利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
In this paper, the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed.
本文实现了重组别藻蓝蛋白基因在毕赤酵母中的异源表达。
Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes.
结论毕赤酵母系统重组表达的膜联蛋白V具有较高的结合外露PS红细胞的能力。
Results By disrupting the PEP4 gene in Pichia pastoris, the productivity of heterologous protein expression increased 9.5% on average.
结果剔除毕赤酵母中的PEP4基因后外源蛋白质表达量平均提高9．5%。
Conclusion Highly-active recombinant mutant human annexin V with endogenous metal-chelating sites can be expressed in Pichia Pastoris system.
结论 利用毕赤酵母系统表达出高活性的、具有内在金属螯合位点的突变型人膜联蛋白V。
Objective:To investigate the effects of different secretion signals on efficiency of onconase(ONC) secretion in Pichia pastoris expression system.
目的：研究不同分泌信号对豹蛙酶（ONC）在巴斯德毕赤酵母中分泌效率的影响。
Objective:To investigate the effects of different secretion signals on efficiency of onconase(ONC) secretion in Pichia pastoris expression system.
目的：研究不同分泌信号对豹蛙酶（ONC）在巴斯德毕赤酵母中分泌效率的影响。
应用推荐