Procyclic trypanosomes were electroporated with linearised plasmid DNA and selected for resistance to phleomycin.
用线性化质粒DNA 电穿孔前环锥虫体，筛选其对磷霉素的抗性。
Plasmid DNA was extracted by alkaline lysis method.
碱裂解法提取质粒。
The plasmid DNA was extracted by alkaline cleavage method.
用碱裂解方法抽提质粒。
Conclusion: Plasmid DNA could influence SR Ca 2 + transport.
结论：外源质粒dna可影响肌质网钙转运功能。
Objective: Study on extraction of plasmid DNA with phenol-chloroform from clone.
目的：对用酚-氯仿直接从克隆中提取质粒dna的方法进行了研究。
Objective To explore a procedure for preparation of plasmid DNA for gene therapy.
目的探索基因治疗用质粒DNA的制备工艺。
Results show that carbon source, nitrogen source affected plasmid DNA yield significantly.
实验结果表明：碳源、氮源对质粒dna产量有明显的影响。
Supercoiling is necessary for the expression of recombinant plasmid DNA at transcription level.
超螺旋化对于质粒dna在转录水平上的表达是必需的。
The transformability of plasmid DNA in B. subtilis competent cell depends on its molecular structure.
枯草芽孢杆菌感受态细胞的质粒转化与质粒的分子构型有关。
Aim: To investigate the effects of injecting plasmid DNA into toad skeletal muscle on its contractility.
目的：应用蟾蜍坐骨神经腓肠肌标本，观察肌内注射质粒DNA后不同时间对肌肉收缩功能的影响。
The extraction results show that the clear and integral plasmid DNA bands were got using these two methods.
结果表明，应用这两种方法可以得到清晰完整的质粒带。
Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material).
开启真空装置使液体通过管柱（质体DNA会与管柱物质结合）。
Objective: To develop an efficient economical and environmental method for the preparation of highly purified plasmid DNA.
目的：建立简便高效、成本低廉和安全无污染的高纯度质粒提取方法。
Plasmid DNA profiles analysis offer scientific reference to molecular epidemiology investigation of avian colibacillosis .
质粒DNA图谱分析为从分子水平上进行鸡大肠杆菌病流行病学调查提供了基础材料。
To study the influence of foreign plasmid DNA on spleen metabolism in immune-stimulated mice via the gastrointestinal tract.
研究外源质粒dna经胃肠道途径对免疫激活状态下小鼠脾脏代谢的影响。
This system was evaluated using plasmid DNA with complete HBV genome and 355 sera from hepatitis patients and normal persons.
采用含HBV全基因组的重组质粒DNA对355份肝炎患者或正常人群的血清样本进行检测，评价该系统的效果。
The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.
采用碱裂解法与DEAE -纤维柱层析法提取与纯化抑制素质粒dna，用分光光度法测定其浓度和纯度。
Objective To prepare plasmid DNA-containing liposomes using detergent removal method and investigate the properties of liposomes.
目的用去污剂透析法制备载基因脂质体并考察其性质。
Objective to study total nutrient admixture (TNA) promoting plasmid DNA transfection mediated with liposomes to colorectal cancer cells.
目的探讨全营养液(TNA)促进脂质体介导质粒dna转染结直肠癌细胞的作用。
Plasmid DNA and chromosomal DNA of the strain CEOI all obtained positive dot-blot to plasmid probe and chromosomal probe correspondingly.
质粒dna和染色体dna分别与质粒探针和染色体探针杂交出现典型的阳性杂交斑。
CONCLUSION: Foreign plasmid DNA, administered via the gastrointestinal tract, may widely modulate the expression of many genes in the intestine in mice.
结论：外源质粒dna通过胃肠道途径可广泛调控肠道多种基因表达。
Methods Agarose gel electrophoresis of plasmid DNA, adherence test, and extract of plasmid were used. Electron microscope photos were prepared and observed.
方法：应用琼脂糖凝胶电泳试验、电镜照片制备及观察、粘附试验及质粒消除试验。
It was observed by fluorescence microscope that plasmid DNA could be delivered into C2C12 cell lines, and the genes were effectively expressed in receptor cells.
通过在荧光显微镜下的观测，证明质粒dna可被导入C2C 12细胞，并且其所携带的基因可在受体细胞中有效的表达。
The effects of concentration of NaOH and SDS temperature and duration of incubation on the isolation and purification of large plasmid DNA of Shigella were studied.
本实验采用碱变性的方法制备痢疾杆菌大质粒。在制备中观察了碱、温度、时间及SDS几个因素对质粒纯度的影响。
In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately.
报道了含sv40复制起点的质粒dna在真核细胞抽提物中进行复制的DNA体外复制系统的建立。
AB-423 showed favourable pharmacokinetic properties when dosed orally in mice and was tested for efficacy in SCID mice that were hydrodynamically injected with HBV plasmid DNA.
而以口服方式给药，AB- 423在小鼠身上也表现出良好的药代动力学特性，也对给SCID小鼠高压注射HB V质粒dna的有效性进行测试。
Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.
方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒dna。
Foreign plasmid DNA was cleared from cellular system in short time after intramuscular injection. Foreign plasmid can not integrate into host chromosome genomic in the immunized mice.
肌注外源质粒后，外源质粒在小鼠细胞内逐渐被清除，外源质粒不会整合到宿主染色体上。
Foreign plasmid DNA was cleared from cellular system in short time after intramuscular injection. Foreign plasmid can not integrate into host chromosome genomic in the immunized mice.
肌注外源质粒后，外源质粒在小鼠细胞内逐渐被清除，外源质粒不会整合到宿主染色体上。
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