To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的克隆人TRAIL基因，构建其原核表达载体。
Objective To obtain recombinant human granulysin using prokaryotic expression system.
目的克隆人颗粒溶素基因并进行原核表达。
So human CT antigen NY-ESO-1 could be successfully expressed in prokaryotic expression system.
本研究成功利用原核表达系统实现了对CT抗原NY-ESO-1的可溶性表达。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
Objective To isolate human arresten gene with Chinese origin for efficient prokaryotic expression.
目的克隆中国人阻滞原基因并高效表达。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
The coating antigen in a 96 pore plate in the kit is the prokaryotic expression recombinant P30 protein, and has good antigenicity.
试剂盒中96孔板中的包被抗原为原核表达的重组P 30蛋白，其具有良好的抗原性。
Methods Different domains of STAT6 were amplified by PCR technique and ligated respectively into the prokaryotic expression vector.
方法：采用PCR技术，体外扩增STAT6分子的不同功能域，扩增后的目的基因分别构建于表达载体。
Objective To modify the HA1 gene of high pathogenic avian influenza virus (HPAIV) and to build an efficient prokaryotic expression system of it.
目的改构高致病性禽流感病毒血凝素基因，建立有效的原核表达体系。
Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen (WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
Construction of the prokaryotic expression vector provided a foundation for further studying the function of NS1 protein and preparation of NS1 antibody.
该表达载体的构建为获得大量NS1蛋白进行功能研究及抗体制备提供了基础。
The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 (DE3), then after, IPTG was added to induce expression.
将构建好的ABP1及TIR1原核表达载体，转化表达宿主菌bl21 (DE3)，加入诱导物iptg进行诱导表达。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体，并且在大肠杆菌中表达。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
Objective to construct the prokaryotic expression vector of human neuronal pentraxin 2 (NPTX2) gene, induce the expression of the recombinant fusion protein in e.
目的在大肠埃希菌中表达人神经元正五聚蛋白2 (NPTX2)，并对该重组蛋白进行纯化、鉴定。
Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的：构建人热休克蛋白70（HSP 70）的原核表达载体，诱导其表达并纯化。
Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.
结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective: to construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation.
目的：构建血管基膜衍生多功能肽克隆和原核表达载体，并对血管基膜衍生多功能肽氨基酸序列进行空间结构分析预测。
Conclusions CBLB502 protein could be successfully expressed and purified using prokaryotic expression system. It has the excellent radiation protective effect in mice.
CBLB502蛋白预防组全部存活。结论成功表达并纯化CBLB502蛋白，并验证其对小鼠的辐射防护作用。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
Conclusion Different molecular weight granulysin were successfully expressed using prokaryotic expression system, which would be helpful for the further study of granulysin.
结论利用原核表达体系成功地表达了不同分子质量的颗粒溶素融合蛋白，为颗粒溶素的后续研究奠定了基础。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
This may be a good method for obtaining soluble and abundant plant actin isoforms and other proteins which could not fold exactly and exist in inclusion body by prokaryotic expression.
这一方法可能是解决植物肌动蛋白及其他在包涵体中表达蛋白难以正确折叠和大量纯化的有效途径之一。
This may be a good method for obtaining soluble and abundant plant actin isoforms and other proteins which could not fold exactly and exist in inclusion body by prokaryotic expression.
这一方法可能是解决植物肌动蛋白及其他在包涵体中表达蛋白难以正确折叠和大量纯化的有效途径之一。
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