The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.
由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。
The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.
由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。
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