蛋白质纯化；
Protein Purification techniques focuses on unit operations and analytical techniques.
蛋白质洗净技术集中于机组营运和分析技术。
Hydrophobic interaction chromatography (HIC) is an attractive method for protein purification.
疏水作用色谱法(HIC)是一种有效的蛋白质分离纯化方法。
The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary.
因此两实际的方法关于蛋白质洗净的书被完全更新并且必要的话重写。
A novel method for protein purification was presented, which was the integration of organic antisolvent and compressed gas isoelectric precipitation.
将有机溶剂抗溶剂与高压气体等电沉淀技术结合起来，提出了一种新的蛋白质纯化方法。
This will help us to investigate controllable protein cleavage in vitro, further establish efficient protein purification system and investigate splicing mechanism of split-inteins.
这将为进一步研究其体外可控性剪接、构建高效的蛋白纯化系统和深入研究蛋白质内含子的剪接机制提供基础。
The results indicate that MALSI- TOF- MS is one of the most effective tools for the assessment of protein purification processes and for the determination of protein molecular masses.
实验结果表明MALDI-TOF-MS法是检测蛋白纯化过程和分析蛋白相对分子质量十分有效的手段。
Therefore, since 1970's, as a new type of functional material, it has gained broad applications in cell separation, protein purification, immunoassays, immobilized enzyme and target delivery of drug.
因此，自70年代以来，磁性微球作为一种新型的功能材料，在细胞分离、蛋白质提纯、免疫测定和靶向药物等诸多领域有着广阔的应用前景。
It was reported here the purification and crystallization of Protein Disulfide Isomerase.
在此报告了它的分离纯化和晶体生长。
This would not have been possible without high-throughput protein production and purification capabilities.
这需要很高的蛋白产量和纯化能力。
First, we learn in Chapter 11 about process optimization and characterization studies for the purification of an E. coli-expressed protein product.
首先，我们在第11章中知道了关于大肠杆菌表达的蛋白产物的纯化工艺优化和特性研究。
Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.
目的探索从骨肉瘤细胞条件培养基中提取骨形态蛋白（BMP）的方法，并测定其生物学活性。
Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.
目的探索从骨肉瘤细胞条件培养基中提取骨形态蛋白(BMP)的方法，并测定其生物学活性。
Analyse the protein difference of purification process Through SDS-PAGE.
PAGE分析纯化过程中蛋白质组成的差异。
The purification of UCP-l(Uncoupling protein -1) from BAT(brown adipose tissue) of the tree shrew.
中缅树?褐色脂肪组织解偶联蛋白质-1的纯化。
The purity of target protein could reach 95% after purification.
纯化后的目的蛋白的纯度可达到95%。
Neuropeptide y; Fusion protein; Inclusion body; Purification; Bioinformatics.
人神经肽y；融合蛋白；包涵体；纯化；生物信息学。
Objective To develop a method for the purification of recombinant human collagen-like protein.
目的建立重组类人胶原蛋白分离及纯化工艺。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The purification procedure was established, and the purified TSF and its fused protein GST TSF were obtained.
建立了TSF蛋白的纯化复性方法，获得了GST - TSF融合蛋白及TSF蛋白。
Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的：构建人热休克蛋白70（HSP 70）的原核表达载体，诱导其表达并纯化。
Objective To establish a method of purification of HPV16L1 protein expressed in a prokaryotic system and obtain the purified protein.
目的建立HPV16 L 1蛋白原核表达系统的纯化方法，纯化目的蛋白。
One of the cruxes of the industrial scale production of protein is its separation and purification, the characters and key problem of which are analyzed.
蛋白质分离纯化是蛋白质产品工业化生产的关键之一。
Protein content and amylase activity of the derivatives of each purification step were determined.
提纯过程中每步都作蛋白质含量测定，并作淀粉酶活性分析。
The paper describes process of recombinant fusion protein in purification and existing problems.
本文论述了基因重组融合蛋白纯化过程和存在的问题。
The techniques for seperation, dissolution and purification of the toxic protein crystal from Bacillus thuringiensis are reviewed in this paper.
本文介绍了苏云金杆菌晶体毒素的分离、溶解及纯化技术。
After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag.
纯化后的的目的蛋白进一步复性，BMP-2经变性液溶解，装入透析袋内。
The induction temperature, induction time, and IPTG concentration were also optimized by a series of experiments. Further purification modes of this protein were also explored.
同时还进行梯度实验分别对诱导的温度、时间和IPTG诱导时菌体浓度进行优化，并对蛋白的纯化方案进行摸索。
Our results showed that, during the spore purification, this protein persistently existed on the spore surfaces with great amount.
我们的试验结果表明，在纯化、洗涤芽孢的过程中，该蛋白持续且大量的存在于炭疽芽孢表面。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
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